| Objective To observe the influence of Astragalus root on Nerve Function after Spinal Cord Injury, and to explore the effect of Astragalus root on treatment after Spinal Cord injury. To provide the oretical guidance for treatment of Spinal Cord injury and give play to the advantages of TCMMethods One hundred and eight female SD rats were randomly divided into SCI group(A group,n=36), Astragalus root treated group(B group,n=36)and MP treated group(C group.n=36).Each group were switch to Allen’s model of spinal cord injury by power of10g×6cm.A group subject to identified quantity of normal saline, and B group receive Astragalus root and C group receive MP. Each group was scored the nerve function by BBB scale at the day before and after injury, and after3d,7d and28d after injury. Before and after operation(1d,3d,28d),somatosensory evoked potential(SEP) was used to assess neurologic recovery of each group. The level of SOD in blood was observed with colorimetric methods at1d,3d,7d,28d after operation. After Id,3d,7d,28d, specimens from injury sites were obtained. Histological changes of injury sites were observed with light and electon-microscope after HE(hematoxylin-eosin,HE) and immunohistochemisty staining of GFAP(Glial Fibrillary Acidic Protein,GFAP). The data were analyzed using statistical software SPSS16.0.Sixty-two Patients’ thoracolumbar veterbra burst fractures between June2011to August2012were strictly selected. In accordance with the order of admission, patients were randomly divided into treatment group (A group) and contral group (B group), two groups of patients were all treated with posterior open reduction and internal fixation of thoracolumbar fractures. After surgry, the patients of contral group were treated with routine therapy of hormone dehydration durg. The patients of treatment group were treated with Astragalus injection on the basis of routine therapy. The two groups were evaluated by ASIA score before and after treatment. The data were analyzed using statistical software SPSS16.O.Results1. BBB scale:On the spinal cord injury day, the hindlimbs BBB scale of A, B, and C groups was0, that was no significant difference between three groups(P>0.05) before3d after spinal cord injury. Over time, motor function of the posterior limbs of rats showed different degrees of recovery. At28d, B group showed higher degree of recovery than the A group(P< 0.05). At7d and28d, C group showed higher degree of recovery than the A group(P<0.05).2. SEP:The latencies of SEP were prolonged and amplitudes were decreased after SCI. Over time the amplitude and the latency improved in various degrees, the latency of A and B groups was no significant difference between two groups(P>0.05) before7d after spinal cord injury. At28d, B group showed higher degree of recovery than the A group(P<0.05). At7d and28d, the amplitude of B and C groups showed difference than A group(P<0.05), B group showed difference than C group(P<0.05).3. SOD:The level of SOD was gradually reduced after SCI. Over time the level of SOD improved in various degrees. Level of SOD of B and C groups recover significantly than the A group (P<0.05). there was no significant difference between B and C groups(P>0.05) at1d and3d, but there was significant difference(P<0.05) at7d and28d.4. Pathohistology:Spinal cord injury resulted in astrocyte acitivation and proliferation and increased expression of GFAP. Expression of GFAP of B and C group reduced significantly(P<0.05), leading to reduction of glial scar, then there was significant difference between B and C groups (P<0.05)at3d and7d.The marks of touch felling, pain felling, movement, and the Neurological Classification of Spinal Cord Injury(ASIA) of two groups showed different degrees of recovery.21days after, A group showed higher degree than B group(P<0.05) in movement and ASIA classification.Conclusion1. Astragalus root can give relief from early decline of activity of SOD, inhibit the lipid peroxidation after spinal cord injury, improve microcirculation and thus showes a promising euro-protective effect.2. Astragalus root can slow expression of GFAP, thus reducing formation of glial scar, promote recovery of nerve function after spinal cord injury.3. Astragalus root can promote recovery of behavior and neural function, alleviate edema of spinal cord, reduce damage of local apoptosis and thus improve the nerve function.4. Astragalus root was conducive to recovery after the surgical treatment of thoracolumbar fractures... |
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