Establishment Of FIA Employed As An Analyzing Approach For AGEs Formed In Vitro And A Novel CAM Assay In Vivo, Together With Screening Chinese Traditional Medicine For Treating And/or Preventing Diabetic Angiopathy

Part 1Establishment of FIA employed as an analyzing approach for AGEs1 Establishment of an analyzing approach for advanced glycation end products formed in vitro by flow injection assayABSTRACT Objective To establish an analyzing approach for advanced glycation end products formed in vitro. Methods Flow injection system was assembled using HPLC to detect AGEs formed in vitro. Other analyzing approaches were employed as controls. Fluorescence of each sample was determined at excitation/emission wavelengths of 370/440 nm as well as 335/385 nm. Results There was a good liner between AGEs concentration (diluted to 0.1-0.0001) and peak area (obtained at 440nm with excitation at 370nm). The equation was y=67978.98x+4.38, 1=0.99999. The coefficient of variance of reproducibility, within-day and between-day assayed by flow injection assay for AGEs was 1.11%, 0.94% and 2.51% respectively. The average recovery rate was 98.5%. There were correlations between flow injection assay and other measures both in data and the results. The data, as well as the results, detected by flow injection was correlated with those detected by other measures. The same results were observed at 335/385nm. Conclusion AEGs detected by flow injection assay shows excellent accuracy and reproducibility. It is a novel method for monitoring AGEs formed in vitro.