Effect Of Quercetin Linosomes On The Formation Of Advanced Glycation End Products (AGEs) And Receptor For Advanced Glycation End Products (RAGE) And TGF-β1 In The Kidney Of Diabetic Rats

Objective: To observe the effects of quercetin liposome(LQ) on formation of advanced glycation end products(AGEs)and receptor for advanced glycation end products(RAGE) and TGF-β1 in kidney of diabetic rats.Methods: LQ was made by rotary evaporation, and the model of type 2 diabetic rats were established by being fed on high-sugar and high-fat diet combined with intraperitoneally injection of streptozotocin(STZ). Then type 2 diabetic rats were randomly divided into six groups: diabetic model group(group DM), low level of LQ group(group LQ-L), medium level of LQ group(group LQ-M), high level of LQ group(group LQ-H), positive control group(group aminoguanidine, AG) and control group(group N). After 8 weeks of interventions, blood glucose, body weight, kidney hypertrophy index(KI), blood urea nitrogen(BUN), serum creatinine(Scr), blood superoxide dismutase(SOD) and malondialdehyde(MDA) were measured in each group. ELISA was used to detect serum AGEs, and 24 h urine albumin. The pathological change of glomerular basement membranes was observed by PAS staining and the expressions of AGEs and TGF-β1 in kidney was assessed by immunohistochemical method. The transcription level of RAGE mRNA and TGF-β1 mRNA in kidney was determined by RT-PCR.Results: Compared with the group N, the level of blood glucose, KI, BUN, Scr, MDA, serum AGEs and 24 h urine albumin were increased significantly in group DM, while the level of SOD activity in serum and body weight decreased. Also the volume of kidney glomerulus increased and glomerular basement membranes thickened, the transcription levels of AGEs, TGF-β1 and RAGE mRNA, TGF-β1 mRNA in kidney tissue increased in DM group(P < 0.05). Compared with group DM, the level of blood glucose, KI, BUN, Scr, MDA, serum AGEs and 24 h urinary albumin decreased, while the level of SOD activity in serum and body weight increased in all three LQ groups. Meantime, the change of pathological morphology of glomerular basement membranes reduced and the expressions of AGEs, TGF-β1and RAGE mRNA, TGF-β1 mRNA in kidney tissue decreased in all three LQ groups. All changes in the medium LQ dose group were more obvious than those of other two LQ groups(P < 0.05).Conclusions: LQ has effect on inhibiting the action of proteinum unenzymatic glycosylation and extracellularmatrix(ECM) hyperplasia while decreasing the production of AGEs, TGF-β1 in serum as well as the expression of RAGE mRNA and TGF-β1 mRNA in kidney. Therefore, LQ play important protective role in kidneys of diabetic rats.