Study On The Molecular Mechanism Of The Components Of Milkvitch-Red Sage Compound Elevating Placenta Blood Supply

Objective: TO explore the molecular mechanism of the components of milkvitch-red sage compound elevating placenta blood supply from the point of view of invading by nutrient cells languishment, formation of placental decidual vas net, endurance mechanism and languishment of nutrient cells.Methods: Separate and abstract the effective components of Huangqi (Omni-SaponuK total-flavone) and Danshen( Danshen-ketone, Danshen-Quinone).Applying L-Arginine methyl ester (L-NAME) to pregnant rats by IP to make Nitrogen Oxide Synthesis blocking models (uterus-placenta-fetus lacking blood and oxygen model or pregnant hypertension syndrome model). On day 12,18 of pregnancy paunched the rats and took out the fetus and placenta/uterus. Measured the basal BP during pregnant period and BP, protein, basal activity each day. Observed placenta/ deciduas ultramicroscopic structure by electron microscope and took count oflanguishment cells. Measured the expressive intensity of bcl-2, Bax of placenta! nutrient cells by SABC method. Checked up the mRNA expression of VEGF> eNOS^ iNOS, MCP-K MMP9 of placental nutrient cells and vas endothelioid cells by FISFLResults: The placental villus of model group were obvious less, languishment of nutrient cells was obvious higher than the other groups(P<0.01).The pathologic damnification of treat group and blank group were superior to the model group.(p<0.01).The expressing of placental nutrient cells bcl-2, Bax of treat group and blank group were significant difference from model group(p<0.01).The mRNA expressing of placental nutrient cells VEGA, eNOS of treat group, blank group and positive group were obvious higher than model group(p<0.01),while MCP-1 > iNOS^ MMP9 were obvious lower than model group(p<0.01).Conclusion: The components of milkvitch-red sage compound restrain the placental immune pathologic damnification and the languishment of nutrient cells of NO Synthesis blocking rats, which is probably due to the accommodation of bcl-2/Bax expression. The components of milkvitch-red sage compound have an effect on elevating placenta blood supply by accelerating the form of placental vas and the establishment of circulation. It can also improve pathologic damnification of placenta and uterine helical artery, elevate vigor of placental nutrient cells and the formation of villus by restraining the immune reaction of placenta. From the approaches above, the components ofmilkvitch-red sage compound can elevate placenta blood supply and restrain the placental immune pathologic damnification to Prevent pregnant compounds such as PHS, IUGR and so on.