| Alcohol has been consumed by human being for thousands of years, and with the increase in alcohol consumption, cases of alcohol-induced osteonecrosis increased correspondingly, the incidence of which is only lower than that of steroid-induced osteonecrosis among nontraumatic osteonecrosis. Patients of alcoholic osteonecrosis are mainly middle-aged or young people with a long history of alcohol abuse, and the liability of deformity resulted from this disease destroys productivity severely, which arouses a wide attention. Due to the controversy of its pathogenesis an effective way of prevention and treatment is especially needed. Therefore, our study was designed as follows:1.Experimental study of setting up the animal models of alcohol-induced osteonecrosis and observing histopathological changes in the models (1) Purpose: To establish a kind of animal model of osteonecrosis induced by alcohol, and to study its pathogenesis. (2) Method: 55 Chinese white rabbits were divided into three groups. The rabbits in each experimental group were treated with distilled spirit (50%, v/v)(10ml/kg/d) and half of the dose (50%, v/v)(5ml/kg/d) respectively, and isodose 50% glucose water for the controls. The animals were killed in batches in the second, the fourth, the sixth and the eighth week. (In the second, the fourth and the sixth week, two rabbits in each of the 3 groups were killed.) The histopathological changes of the bilateral hip joints and the humeral heads were observed radiographically before and after the treatment; the animal hearts, livers, lungs and kidneys and musles were obtained to be observed under a light-microscope, and histopathological changes of the bone samples of the proximal/distal of the femur and the proximal of the humerus were observed under light and transmissible electron-microscope. According to the histological appearance, the incidence ofosteonecrosis of each group was calculated. Bone samples of each period were observed under high-power microscope to note the percentage of empty osteocyte lacunae. All of the data were statistically analyzed. (3) Results: The incidence of osteonecrosis of the group treated with whole dose was obviously higher than that of the half-dose-treated group (83.3%Vs63.6%). Histopathologically, the necrotic foci were mainly observed in the subchondral region of the femoral head, the hematopoietic tissue of bone marrow was decreased, and vacuolar disfiguration were observed; the trabeculae became thinner and sparse, accompanied with bone resorption, and empty osteocytes lacuna in the trabeculae were increased. (4).Conclusion: The animal models of alcohol- induced osteonecrosis can be set up successfully by giving a high dose of distilled spirit, and the incidence of necrosis is correlated with the dosage.2. Significance of study on blood chemistry, blood coagulation and fibronbosis with regard to alcohol-induced avascular necrosis of femoral head.(1). Purpose: To investigate the high risk factors of avascular necrosis of femoral heads induced by chronic alcohol consumption, and to look for an effective method to screen the high risk group of alcohol-induced osteonecrosis,. (2). Method: 23 animal models treated with alcohol (the detailed method was the same as part 1) were used, and blood samples from the heart with empty stomach were serially collected immediately before and after the final treatment. Serological tests containing lipid component analysis (CHOL, TRIG, HDL-C, LDL-C, Lp(a)), liver function( ALB, ALT, AST, ALP), the coagulation index ( APTT, PT, TT, Fbg ), and the index of pre-thrombus condition (PAI, D-Dimer, t-PA, AT-III) were performed. Finally the animals were divided into two groups according to the result of histopathological obvservation: Group ON (osteonecrosis), and Group NON (no osteonecrosis). The indexes described above before and after the experiment were compared between these two groups. (3). Results: the level of serum lipid (CHOL) rose significantly in each group (P<0.01), especially in group ON, which was significantly d...
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