The Change Of Energy Metabolism In The Region Of The Perihemotoma After Brain Hemorrhage

Part I [Objective] To observe the change of the energy metabolites and theactivity of the key enzymes of energy metabolism in the perihematomaregion in rabbit brain after intracerebral hemorrhage. [Method] The rabbits were divided into 3 group: normal group(N),hemorrhage group (ICH) and sham group(SH). The ICH and SH groupwere subdivided into 1h, 6h, 12h, 24h, 48h and 72h group. The model ofcerebral hemorrhage in rabbit was made with autologous blood infusion.Content of water, ATP, ADP, AMP and lactate in perihemotoma region weredetermined, and the metabolite concentrations in edematous brain regionswere corrected for dilution. The value of EC was calculated through the valueof ATP, ADP, and AMP which were corrected. The activity ofphosphofructokinase (PFK) and H+－ATPase were assayed too. [Result] There was obvious edema in perihemotoma region at 1 hourafter ICH and more seriously to 72 hour. ATP decreased moderately at 1hour, and remain the similar level until 12 hour. There was another markeddecrease at 24 hour, whereas there was no obvious change from 24 to 72hour. ADP content began to decrease at 6h (P<0.05) and reached the lowestlevel at 24h. There was no difference between ADP content of 24h and72h. The change of EC value shew the rule similar to the change of ATPcontent. The content of lactate increased in 1 hour and reached the peak at12 hour. Until 72 hour , the level of lactate in hemorrhage group was higherthan that of N and SH group. The activity of phosphofructokinasedecreased markedly at 1 hour and remain the tendency of decreasing to 72hour(P<0.05). The activity of H+-ATPase was elevated lightly (P>0.05),after that it shew the continuous decreasing and had the obvious differencewith N and SH group from 12h. [Conclusion] The failure of energy metabolism taken place in 24hour after the ICH which delayed to the brain edema. The results may berelated to the change of some enzyme which take key role in energymetabolism.Part II [Objective] To investigate the effect on the injury of perihemotomaregion by the energy metabolism failure and the influence by 1, 6-biphosphatofructose. [Method] The rabbits were divided into 4 group: normal group(N),sham group(SH),hemorrhage group (ICH) and treatment group (TR). TheSH , ICH and TR group were subdivided into 1h, 6h, 12h, 24h, 48h and 72hgroup. The model of cerebral hemorrhage in rabbit was made withautologous blood infusion. The content of ATP the LA, the activity of PFK,the NSE level in serium and the change of aptosis in perihemotoma regionin all groups were compared. [Result] There was no difference in all groups at 1, 6, 12h. At 24h,48h, 72h ATP content of TR group was higher than that of ICH groupwhereas lower than that of N and SH groups (P<0.05). At 1h, 6h, 12h,compared with ICH group the increasing amplitude of LA was lowered(P<0.05) whereas at 24,48,72h there were no differences between ICH andTR groups. The peak of LA was delayed to 24h. But all the time the levelof LA of ICH and TR groups were higher than that of N and SHgroup(P<0.05). The content of NSE in serium showed the increasingtendency from 6h (P<0.05). After the treating with FDP, the content of NSEof TR group was lowered than that of ICH group obviously (P<0.05) andhad no difference with that of N and SH group(P>0.05). After 24h, thecontent of NSE in TR group was lower than that of ICH group and stillhigher than that of N and SH group(P<0.05).There were few aptosis cells at6h postinfusion, the number of aptosis cells increased markedly at 24h, andthere were a lot of aptosis cells at 72h. Treating with FDP, the number ofaptosis cells in all time was less than that of ICH group. [Conclusion] 1.FDP could improve the energy metabolism . 2.Therewere obvious injury in the perihemotoma region. 3. The injury wasameliorated after the improvement of energy metabolism. S...