| Objective: To investigate the mechanism of Shenshuai Capsule delaying evolve of chronic renal failure and interstitial renal fibrosis by observing the renal function, routine examination of blood, extracellular matrix and cytokine expression in clinical and experimental study. Methods: Seventy four patients with chronic renal failure in azotemia period were divided randomly into treated group and control group which were respectively treated with Shenshuai Capsule and Uremic Clearance Granule. We observed the changes of BUN, Scr, Hb, RBC, ColIV, PCIII, LN, uPro, 2-m in blood serum and improvement of clinical symptom and physical signs. In the experimental study, we took the model of interstitial renal fibrosis in chronic renal failure caused by adenine. Changes of BUN, Scr, ColIV, PCIII, FN in blood serum were observed; expression of I, III type collagen protein, CTGFmRNA and TGF- 1mRNA in nephridial tissue were examined respectively by immunohistochemistry with two steps, in situ hybridization and semi-quantitative RT-PCR. Results: After treated by Shenshuai Capsule, BUN and Scr of patients of CRF declined, Ccr, HB and RBC raised, ColIV, PCIII, LN, uPro, 2-m in blood serum declined which had obvious difference with control group. The clinical symptoms and phydical were obviously improved. Shenshuai Capsule could reduce BUN, Scr in rat blood, and relieve I ,m type collagen protein expression in nephridil tissue. It could also inhibite the CTGFmRNA and TGF- 1mRNA expression and reduce the content of ColIV, PCIII in blood serum. Conclusions: Shenshuai Capsule can improve renal function and delay chronic renal failure by reducing BUN, Scr and rising Ccr inblood serum. It can interfere in interstitial renal fibrosis by inhibiting the CTGFmRNA, TGF- 1mRNA expressions in nephridial tissue and reducing I ,III type collagen protein in extracellular stroma and deposit of ColIV, LN.
|
PDF Full Text Request