Proteomic Analysis Of The Therapeutic Effects Of Fufangdanshenfang On Atherosclerosis

Chinese Traditional Medicine can affect signal molecular groups through chemical molecular groups to restore balance of signal molecular networks to cure diseases. The technical system composed by proteomics, gene chip, RT-PCR and molecular blotting was established, and this system could combine signal molecular groups to chemical molecular groups. The effects of Fufangdanshenfang, effective ingredients and combined ingredients to cure atherosclerosis were investigated. The goal of this research was to discover the ingredients and pathways of Fufangdanshenfang to cure atherosclerosis.Proteomic protocol involved of 2-DE, image analysis and spectrometry detection was set up. Compared with coomassie blue and silver staining, DIGE has high detection sensitivity, high accuracy and good reduplicate. Silver staining was fit to ascertain protein spot in 2-DE gels, coomassie blue was fit to detect high content protein, DIGE was fit to establish difference protein expression map. The increased production of NO, PAI-1, and the decreased production of growth factor such as VEGF, bFGF, EGF, TGF-J31 and adhesion molecules such as ICAM1, VCAM1, E-selectin might be the mechanism of ginsenoside Rgl to protect HUVECs from TNF-a induced injury. The expression of G-protein coupled receptor kinase, PKC and CaMKK were decreased by Fufangdanshenfang, which farther decreased matrix metalloproteinase production, and oxidation and inflammation related protein were also decreased , while cell cycle related protein p21, p53 were increased by Fufangdanshenfang in TNFa treated VSMC. The levels of fibrinogen and Granzyme C were elevated and the time of coagulate blood was prolonged in Fufangdanshenfang treated plasma. The changes of protein expression map, gene expression, and the time of coagulate blood were similar betweenFufangdanshenfang and its combined ingredients, and the changes of protein expression map was different between Fufangdanshenfang, Ginsenoside Rgl and Tanshinone II A, thus it could be seen that the effects of combined ingredients were similar to the effects of Fufangdanshenfang.Conclusions: The main factors which play important roles in the mechanism in the pathogenesis and development of atherosclerosis could be summarized as the following: dysfunction of vein endothelial cells, proliferation and migration of VSMC, weaken of fibrinolysis, strengthen of oxidation and inflammation. The effects of Fufangdanshenfang on atherosclerosis included that: protection of vein endothelial cells, inhibition of proliferation and migration of VSMC, regulation of fibrinolysis and coagulate, weaken of oxidation and inflammation.