Mechanism Study Of Wild Type P53 Gene Concomitant With Oldenlandia On Primary Liver Carcinoma

Objective:To explore the mediated effects of tumor suppressor gene wild-type p53 and Chinese traditional medicine oldenlandia on primary carcinoma of liver and research the new method against tumor of combining tumor suppressor gene and Chinese traditional medicine. Methods:Animal mode of mice with primary carcinoma of liver was estabalished and the animal were divided into four groups randomly.hepotacarcinoma group(group I ) ;Gene therapy group(group II) : wild-type p53 adenovirus liquor(0.2ml) was injected to the tumor area once a day for 12 days;Group III:Gene and oldenlandia group,each animal in this group was perfused with oldenlandia decoction (lmg/ml)1.0ml/day and 0.2ml wild-type p53 adenovirus liquor injection at the tumor simultaneously for 12day and Oldenlandia group(groupIV),in which mice was perfused oldenlandia decoction(1.0ml/day) for 12days.Then the telomerase activity of tumor,cell apoptosis,bcl-2\PCNA expression and immune funciton markers were detected by corresponding methods. Results:The tumor tissue is notably diminished in size with the treatment of wild-type p53 and minished more significantly at the Gene and oldenlandia group.The telomerase activity is deregulated obviously at the Gene group and Gene oldenlandia group,and many apoptotic cells can be observed.The tumors treated with wild-type p53 or wild-type p53 plus oldenlandia showed that presence and expression of apoptosis-mediated protein bcl-2\PCNA were demonstrated using irnmunohistochemistry.that bcl-2\PCNA positive cells showed the accordant distribution with the apoptotic cells.The T-lymphatic cells in mice perfused with oldenlandia decoction display the salient reaction to antigen stimulation and the amount of T cell antigen receptor is increased concomitant with the prominent excretion of serum cytokine IL-2 and GM-CSF.The perform mRNA expressionwas affirmed in hepatocarcinoma tissues in Gene group and Gene plus oldenlandia group assessed by RT-PCR,whereas nontreated cells didn't show these changes. Conclusions: Wild-type p53 can significantly inhibit the growth of hepatocarcinoma, and the inhibiting function was enhanced with the use of oldenlandia;Wild-type p53 repressed hepatocarcinoma growth through restraining telomerase activity, deregulating bcl-2 and PCNA expression and inducing cancerous cell apoptosis and oldenlandia can reinforce the immunologic reaction through enhancing the T-lymphocytic conversion function,expression of TCR receptor,IL-2/GM-CSF excretion and elevating the expression of perforin mRNA.The study suggested that wild-type p53 and oldenlandia had synergistic effect in both inhibiting hepatocarcinoma cell growth and sensitizing the immunologic reaction in vivo to cancerous cells.