The Role And Signature Of Glucose Metabolism Imaging Diagnose The Myocardial Viability

cardiovascular disease is called the first murderer of human health for its higher mortality and morbidity. Coronary heart disease is one of the major causes of death in the world. The ischemia myocardium can lead to the change of the myocardial contraction function even threaten to the patient's life. But patients can recovery and have a better prognosis after promptly correct treatment.Coronary artery lesion can lead to vary outcome with the extent of lesion and the stenosis. Stenosis of coronary artery and reopen of the emphraxis vascular can lead myocardial cell long hypoperfusion which make them have low metabolism and left ventricle functional decreasing slowly but construction of myocardium unchanged, means myocardial hibernation, which main clinical show the segment hypoperfusion, asystole or dysfunctional. Myocardial perfusion or wall motion can recover regional and global after revascularization. The acute ischemia caused by coronary artery acute completely emphraxis can stimulate the myocardium, call myocardial stunning, changing its construction, function and metabolism, which recovery difficult and slowly. Necrosis myocardial is the infarction or scar cause by the serious and longtime ischemia, which means irreversible damage, left ventricle functional not improving even revascularization. Percutaneous transluminal coronary angioplasty has become the preferred method for treating coronary artery stenosis. However, PTCA screen is important for myocardial hibernation and stunning can recovery after therapy. Therefore, assessment myocardial viability has play an important role in clinical. Among many methods evaluating myocardial viability, 18F-FDG PET is the gold standard but the clinical application hampered by the limited availability and high cost of PET and cyclotron technology. Coincidence imaging posses PET function, can it have the same effect of evaluating myocardial viability? The topic perform mainly heart cell uptake and transport 18F-FDG, utilize animal performing basic research with coincidence imaging, detecting the patients so that coincidence imaging can utilize broaden in clinical. The results of this study can provide guide for clinical trials and applications.PartⅠ heart cell uptake the 18F-FDG and mechanism The aim of this part research is to observe myocardial cell in vitro uptake 18F-FDG in various glucose substrate in order to investigate the mechanism of myocardial cell uptake. hearts from 1- to 3-day-old Sprague-Dawley rats were removed to the sterile free Ca2+,Mg2+ Hanks solution. The ventricular cells were dispersed by digestion 5 times with 0.1% pancreatin at 37℃ on the homeothermia magnetism stir instrument. Solutions was collected for centrifuge 1200rpm 5 minute,sedimentations is added to minimal essential media, The cells were preplated for 1 hour to enrich the culture myocytes isolate the fibroblast. Counting the cell density about 1×106/ ml and cultured in media consisting of Hanks' salts plus minimal essential media. Cells are divided into 4 group: low glucose, high glucose and insulin 1mg/L. all the group cell uptake the 18F-FDG ( 7.4MBq/ml) 100μl and collect the cell to detecting radioactivity on the γ-counter and plotting the uptake curve. Cell then is isolated membrane protein with RIPA buffer, protein assays was performed with Bio-rad DC Protein assay KIT. The membrane protein were subjected to sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis and the resolved proteins were electrophoretically transferred to nitrocellulose. The blots were blocked with 5% nonfat dried milk and probed with rabbit anti-mouse GlUT4 (1:200 dilution), and bound IgG detecting horseradish peroxidase-labeled affinity goat anti-rabbit IgG (1:10,000 dilution). Enhanced chemiluminescence detection was performed with X-ray film. The myocardial cell can uptake the 18F-FDG within 50 minutes. The high glucose insulin have the most GLUT4 content, facilitate 18F-FDG uptake, insulin also play the same role to enrich the GLUT4. Part Ⅱ Establish and identify in...