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Anticancer Effect And Mechanism Of Selenium And Building The Model Of Mice Bearing The Prostate Cancer

Posted on:2006-06-27Degree:DoctorType:Dissertation
Country:ChinaCandidate:L WangFull Text:PDF
GTID:1104360152492434Subject:Prevention of Veterinary Medicine
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This experiment investigated the effect and mechanism of selenium compound by using radiation oxidational trauma mice model, chorioallantoic membrane chicken model and ICR mice modle bearing EAC and cells with prostate cancer, and observed the effect of the use of selenium and VCR at the same time to ICR and LNCaP cancer cells. Three SCID mice models—hominine LNCaP subcutaneous tumour, LNCaP prostate tumour and LNCaP subcutaneous tumour castrated are successfully built by using sever combined immunal deficince mice. The following are the results:1. Na2SeO3 and SeMC have the function of protecting the mice suffering radiation oxidational trauma. When mice are radiated with 4.5G radiant ray, their body will be severely damaged by active oxygen radical. After taking the Selenium compound of Na2SeO3 and SeMC, the mice's level of GSH-Px is highly improved, their survival period is prolonged, the quantity of leucocyte in blood is increased, the rate of deformed testicle sperm is lowered, the content of SOD, GSH and GSH-Px is obviously increased compared with the radiant group. This may be because selenium compounds can help clean the active oxygen radical produced after suffering radiant trauma in time, and supply GSH-Px enzyme with enough selenium in time, thus the trauma of organism is decreased.2. Na2SeO3 and SeMC has inhibiting effect to the forming of local angiopoiesis in chicken embryo when the dosage of 20μg/chicken embryo drugs-mythylfibrin membrane is taken. The difference is obvious compared with the control group(P<0.05). But function mechanism needs further investigation.3. When only the dosage of 4μg/gbw Na2SeO3 and SeMC has effect on EAC in ICR mice, the mice's living period is not obviously prolonged. But they can effectively inhibit the growth of cancer cells, make the rate of G1 period cells and the level of SOD, GSH and GSH-PX in serum increase ,and the MDA in serum decrease , hint at the increase of the organism's antioxidant level. When SeMC and VCR are both used, their effects to EAC cells are stronger, and the growth of cancer cells can be obviously inhibited. When 2μg/gbw MSeC and 0.4μg/gbw VCR are both used, the content of SOD and GSH in serum is obviously increased and MDA level is decreased. It represents that the organism's level of antioxidant trauma is increased. Flow Cytometry(FCM) finds out that the use of SeMC and VCR at the same time facilitates the death of cancer cells and prolongs mice's living period. It proves that SeMC and VCR have good coordinated roles in the aspect of resisting EAC tumour.4. VCR has the effect of anti-LNCaP cell. It can induce the LNCaP cell to die. The G2/M is the central cell cycle point effected by VCR and it causes the rate of G2 tumour cells to increase. The combination of Na2SeO3+VCR and MSeA+VCR hasstronger effect on LNCaP tumour cells. The rate of inducing cells to die is greatly increased (P<0.05) and the rate of S-period cells during growing period is decreased . So the combining use of selenium and VCR has coordinated role in resisting LNCaP cancer cells.5. The mice castrated model bearing LNCaP is successfully built by extirpating the double spermaries . After the double spermaries are extirpated, the growth of LNCaP tumour is lowered, even stopped. The concentration of prostate special antigen albumen is rapidly decreased, the quantity of tumour cells in growing condition is sharply reduced. Flow Cytometry(FCM) shows the rate of growing cells is obviously decreased. The cancer cells' mortality is increased. TUNEL detect shows that the quantity of positive cells in the cancer cells is obviously augmented compared with uncastrated mcie and the difference is significantly ( P<0.05 ) . Observation by electrical microscope finds that a great many of cancer cells are in the apoptosis state. A great quantity of dark chromatin appears around the karyotheca, lots of apoptosis body are in the tumor cells. The above results show that female hormone has obvious supporting role to the growth of LNCaP tumour. It is also observed that when LNCaP tumour cells dep...
Keywords/Search Tags:Selenium, PSA, LNCaP, SCID, Flow Cytometry, Apoptosis, Angiopoiesis, Antioxidation, Cell Cycle, VCR
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