The Study Of Chinese Herbal Medicine To Interfere Toxicity On Reproduction And Nervous System Correlating With Pyrethroid

Objective1. The study employed deltamethrin (DM) to jeopardize pregnant rats modeling as the experimental object. It was observed and analyzed about how pyrethroid (Py) affected the pregnant SD rat itself, the filial generation and the reproductive capability. In prospect to those outcomes, we could evaluate pyrethroid based on the outcome of pregnancy and embryonic defect. In addition, the study could explore the mechanism of action towards reproductive system.2. The study used Formula Zhuyun III decoction as the treatment to benefit kidney and spleen. It applied deltamethrin to interfere abortion and embryonic defect in order to theorize the precaution and protection of Chinese medicine and its mechanism of action.3. On the other hand, the study could assess Chinese herbal combined decoction to act on mother-infant protection. It also provided a theoretical evidence emphasizing Chinese medicine approaching reproductive science.Method1. The experimental study of Chinese herbal medicine affecting pregnant rats being poisoned by deltamethrin (DM) with toxic response: 72 SD rats were divided into six groups including (1) model group, (2) modelplus high dose treatment, (3) model plus middle dose treatment, (4) model plus low dose treatment, (5) model plus taurine , (6) Blank control group. Group 1-5 were orally fed l/20LD₅0 (6. 93mg/kg) DM daily from Day 1 to 15 when proved pregrancy. Then, physiological saline was given after being poisoned after 4 to 6 hours. The quantities were 4 times, 2 times, 1 time of adult dosage of Formula Zhuyun III and taurine (0. 2g/kg) . Group 6 was blank control. Half pregnant rats were killed on the fifteenth day. The rest was kept until labour. The experiments were to observe their general condition, weight, pregnancy symptoms, spleen, ovary and uterus, number of embryo and its development. Blood tests were carried out in the mid stage of pregnancy and delivery respectively to generate data of β — endorphone, estradiol, prostaglandin and IFN— γ, IL-2 IL-4 IL-10.2. The experimental study of Chinese herbal medicine to affecting embryonic development whilst maternal rats being poisoned by deltamethrin (DM): The embryos were taken from the fifteenth day pregnant rats. The study observed the embryonic shape and defect; weight, length, tail, and RNA isolation and PCR test of Pax3 and Cx43mRNA. Embryonic brains were examined by Cx43 protein test under immunofluorescence test.3. The experimental study of Chinese herbal medicine affecting infant' s growth whilst maternal rats being poisoned by deltamethrin (DM): The infant rats were observed with their growing conditions, sex proportion, life and death rate. The growing conditions included fur growth, ears and eyes opening, testis and vaginal opening for assessment. Forearm reaction, turn-around reaction, up and down reaction were also being assessed. The observation also tested learning skill and memory ability.4. The experimental study of deltamethrin (DM) affecting the level of brain NO concentration and NOS of the infant rats: On the fourth day and tenth day afer the infant rats were given birth, 12 infant rats were randomly drawn from each group, devitalized. Then, the whole brain was taken, the blood was bleached and the meninx was peeled, physiological saline was applied to bend and grind 10% of brain tissue, estimated NO level and NOS activation was estimated.5. The experimental study of Chinese herbal medicine affecting genital system and the level of endocrine: On the sixtieth day afer the infant rats were given birth, (1) six male rats were taken randomly and six female rats in anestrus were taken by using vaginal test. Sera FSh\ LH T of male were examined. E₂ P FSH LH of female were examined; (2) genital organ was examined and weighed including testis, ovary and uterus; (3) applied Blazak method to obtain sperm count and sperm growth rate (4) immunofluorescence method was applied to assess ovarian protein expression.Results1. Pregnant rat could not find any sign and toxic symptom duringearly/middle pregnancy after being poisoned by l/20LDM deltamethrin (DM).2. With respect to abortion rate, model group showed statistical significance when compared with other groups. Model group also showed progesterone level was low, Thl/Th2 came closely to Thl type. Formula Zhuyun DI is a formula to enhance kidney and spleen. With the treatment Formula Zhuyun HI, it had desirable alleviating effect in abortion rate, higher level of progesterone, and desirable effect to regulate DM preventing from deviation of Thl and TH2. Taurine Group maintained high rate of abortion, but progesterone could not remain high level. The result demonstrated that DM had toxicity on reproduction, it could leadto abortion which was related to endocrine and immune function for pregnant rats. Formula Zhuyun III could regulate the above condition and relief DM toxicity. Taurine could not influence DM to prevent abortion.3. The labour time of all groups was within the normal range. The study showed no significant difference in DM factor.4. Growing condition after being poisoned by DM: Growing rate dropped, length, weight and tail significantly fell. It implied that DM toxicity might transfer to embryo through placenta to induce the side effect. Regarding the rate of life, middle and high dosages were high, high dosage found significant difference in their health condition. However, there was no difference in low dosage. It showed that the positive correlation existed.5. Brain tissue of Cx43 protein expression was stronger when being poisoned by DM. The result indicated that there was positive correlation according to different concentration of Formula Zhuyun III. The higher the concentration, the higher efficiency. Cx43 protein expression fell in taurine and blank control group. The level of Pax3mRNA and Cx43mRNA of embryonic could not show the same trend as the Cx43 protein expression of brain tissue.6. Health condition: all groups were dysfunctioned by DM except blank control group. It implied that DM could affect infant rats' development.7. The early development of infant rats reached to normal growth. But the fur, open ears, male testis drop of blank control group was better than other group.8. Compared to the blank control group, the sex proportion, life rate of all group poisoned by DM had no significant difference.9. DM could affect NOS activation and NO level or infant rats. The brainNOS activation and NO level of the fourth day infant rats in all group poisoned by DM were much higher than blank control group. However, there was no neural toxic symptoms and signs.10. Pregnant rats contacted DM would affect the filial generation which was reflected through endocrine. There showed that male infant rats were affected more obvious relatively. To male, the level of estosterone and production of sperm fell. To female, uterus deficiency was discovered. There implied that endocrine might be affected by DM. Formula Zhuyun III could improve the drawback through the regulation of endocrine.Conclusion1. DM is a typical medicine which proved its reproductive toxicity leadingto pregnant defect and abortion. It could affect the embryo and its development. The endocrine of pregnant rats was affected. However, no neurotoxicity of DM could be found regarding the filial generation.2. From the view point of Traditional Chinese Medicine, DM , one of the factors in the environmental pollution, was treated as a kind of toxin. It could affect the embryo and pregnant mother among dysfunction of organ, imbalance of Yin-yan and damage of reproductive system which could lead to abortion. The infant might lead to deficiency of genetic factor and slow development.3. Formula Zhuyun HI strengthened kidney and spleen to resist DM toxicity. Thus, the abortion rate fell obviously. It could also accelerate the infant development and the reproductive performance of filial generation. It might be caused by the regulation of endocrine and immune system. According to the results, we believe that the medicine with strengthening function of kidney and spleen had a great potential to interfere ECDs reproductive toxicity and to avoid pregnant abortion and embryonic defect.