The Clinical And Empirical Study About The Theory Of The Relationship Between Spleen Governing Movement And Transformation And Bioblast Cytochrome Oxidase

Objetcive : The theories of spleen governing movement and transformation is an important part of the Spleen - stomach Doctrines .It is a complicated process that transform and create the nutrient qi , defensive qi, qi, blood, the fluid ...etc in human body, it involve five zang viscera and six fu viscera, inborns, acquired., but all depend on stomach and spleen to carries the essence of food and drink .Therefore only the spleen governing movement and transformation runs well, can the human body make material metabolism, energy metabolism to maintain normal activity.The so-called internal organs of the Chinese medicine contains the idea of the whole, the function activity of the internal organs, is a series to synthetical activities of the whole. The modern research discovers, the " spling" of the Chinese medicine is not only whole digestive system to of Western medicine, but also multi-systems, multi-function unit, having close contact with nerve, endocrine, blood circulation, immunity, urinary, procreation , sport system etc. As early as 80's, professor Liu you zhang, take the lead to put forward that the spleen governing muscles haves close relation with cell chondriosome. In proceed National"75" Studying task "syndrome of deficiency of spleen mechanism research", observed the variety proceed of gastric mucosa cell chondriosome of sufferers with syndrome of deficiency of spleen by electron microscope. Firstly reports the gastric mucosa cell chondriosome of sufferers with syndrome of deficiency of spleen reduced the ultrastructure is damaged, and energy metabolism is blocked, put forward the theory of spling in Chinese medicine with cell chondriosome. Putting forward the spleen governing movement and transformation, is not only digesting food in the stomach and intestines to absorb , more important is that nourishment material be oxidized to produce energy in chondriosome, making the appetite research into the second cell level firstly.The cytochrome oxidizing enzyme , is the respiratory chain complex IV , locating the bitter end of respiratory chain, catalyze the oxidation of cytochrome, driving at the same time synthesizing the ATP , is the indicating enzyme of chondriosome, its activity is very important to maintain the construction and function of chondriosome and cell energy creation. The energy metabolism is the process of the energy releasing, transfering and using of the material metabolism in organism , it has closely relation with the theories of "spleen governing movement and transformation* of Chinese medicine. This research aims at the relation between 'spleen governing movement and transformation* and chondriosome and the cytochrome oxidizing enzyme to post out such relations.Method: The research consists of two parts: literature review and experiment study. In foundation literature research, firstly study on the function characteristics of *spleen governing movement and transformation* and its influence to the organism and the pathologic diversification, further study on the relation between *spleen governing movement and transformation* and and the cytochrome oxidizing enzyme, demonstrate the relation of 'spleen governing movement and transformation* and energy metabolism in organism;Secondly, overview the history origin, the coming on characteristics, the cause of disease, pathologic mechanism, dialectical point and treatment key of spleen flaccidity-syndrome;define the special relation of spleen flaccidity-syndrome and 'spleen governing movement and transformation* ;At the same time, summarize the characteristics of the ultra structural pathologic variety of flaccidity-syndrome patient.In animal experiment research, for inquirying into the relations between and chondriosome and the cytochrome oxidizing enzyme , set up the rat medel of chronic spleen deficiency, detect chondriosome of animals' skeletal muscle , liver> stomach and heart, cytochrome oxidase's content and activity and its genie change.In research on pathologic check to muscle of patient with flaccidity-syndrome, to make clear the pathologic characteristics of the muscular tissue of the patient with flaccidity-syndrome when they are spleen flaccid, we make specimen of muscle tissue of patient with spleen flaccidity, observe by normal light microscope and electron microscope.Effect: In animals experiment study, the results shows, the rat with spleen deficiency's cell chondriosome ,the content and activity of cytochrome oxidase of skeletal muscle , liver-, stomach and heart decline in different degree (P<0. 05orP<0. 01) .It shows that when animal with spleen deficiency and spleen governing movement and transformation go wrong, the animal's organ's chondriosome and cytochrome oxidase go wrong,...