Research On ICC Characteristic Of Gastric Movement Obstruction Caused By Spleen Deficiency Syndrome And Treated By Chinese Medicine Of Strengthening The Spleen

ObjectiveThe research made gastric movement' s pacemaking cell and Interstitial Cell of Cajal (ICC) as the incision, probed into the pathological mechanism of gastric movement obstruction caused by spleen deficiency syndrome from the form and function of ICC, electricgastro- movement, the gastric emptying movement. It also can provide clinical and experimental proof for XiangSha Six Mild-Drug Decoction to treat spleen deficiency syndrome. Method The section of documentary research:Reviewed partly the understanding of domestic and foreign scholars on gastrointestinal movement obstruction caused by spleen deficiency syndrome in the past 20 years, in addition, summarized the study methods of gastrointestinal movement, the relationship of ICC and gastrointestinal movement obstruction disease, TCM therapy on the disease, the status quo of regulating mechanism of gastrointestinal movement obstruction disease, provided future views of the research. Clinical research:selected 60 patients suffered from the spleen deficiency syndrome accompanied gastric movement obstruction, then divided them into different syndrome group according to the TCM' s diagnostic standard composed bysymptoms, tongue condition and pulse condition, etc;divided the cases into Chinese medicine group and Western medicine group, otherwise, took 10 normal persons as control group. In Chinese medicine group, XiangSha Six Mild-Drug Decoction was used: Rhizoma Atractylodis Macrocephalae (Baizhu)15g, Radix Glycyrrhizac (Ganchao)lOg, Rhizoma Pinelliae(Banxia)15g, Radix Codonopsis (Dangshen) 15g, Pericarpium Citri Reticulatae (Chenpi) lOg, Poria(Fuling) 15g, Radix Aucklandiae (Muxiang) lOg (decoct later), Fructus Amomi (Sharen) lOg (decoct later), one dose one day. Used 500ml water, decocting 200ml decoction, the patients had it half an hour before the breakfast and supper, 4 weeks as a period of treatment. In Western medicine group, Mosapride Citrate Tablets was used. One tablet once (5mg/tablet) , 3 times one day, the patients had it 20 minutes before 3 meals, 4 weeks as a period of treatment. From inspecting day, the patients were asked to avoid tobacco, wine, tea and acrid and fried things, stoppped using any medicine which could affect curative effect. Used the method of symptom classification and fix quantity to evaluate the relieving ratio of spleen deficiency syndrome, adopted X-ray prevention marked substances (Barium Slip Method) to measure gastric emptying ratio, adopted body description method to record Electrogastrogram, In the end, observed the change of indexes mentioned above, which was recorded from the patients suffered from spleen deficiency syndrome before and after the treatment. Experimental research:SD model rats suffered from spleen deficiency syndrome were used as objects, adopted gastric emptying detection, electron microscopic, immunity organization chemistry and RT-RCR techniques, etc. Before and after giving XiangSha Six Mild-Drug Decoction to the rats, observed the change of gastric motility. Form and distributing characteristics of ICC gastric pace-maker unit, gene-expressing variety of ICC cell marked substance c~kit.Single factor variance was adopted to analyze the dates. Between the group' s compare, SNK technique was adopted, the research analyzed the relationship between index and syndrome differentiation, discussed the segmental pathological basis of spleen deficiency syndrome that causing gastric movement obstruction and regulating mechanism of Chinese medicine.Achievement Clinical research:l.The relieving ratio of alimentary tract symptoms of patients suffered from the spleen deficiency syndrome of chronic gastritis.In the relieving ratio of alimentary tract symptoms of the patients, the XiangSha Six Mild-Drug Decoction group was higher than that of Western medicine group, there was obvious difference between them(P<0. 05).2. The gastric emptying ratio of patients suffered from the spleen deficiency syndrome of chronic gastritis.(1) In the gastric emptying ratio before treatment: there was obvious difference between subject group (Chinese medicine group and Western medicine group) and control group (P<0. 01).(2) In the gastric emptying ratio after treatment: there was no obvious difference between subject group (Chinese medicine group and Western medicine group) and control group (P>0. 05), there was obvious difference to the gastric emptying ratio before treatment (P<0.01).3.EGG analysis of patients suffered from the spleen deficiency syndrome of chronic gastritis .(1) In stomach electrical rhythm analysis before the meal:Comparing with control group, the regular slow wave of the Chinese medicine group and Western medicine group reduced obviously (P<0. 01), over-slow rhythm increased obviously (P<0.01), over-fast rhythm has no obvious difference (P>0. 05), Comparing with the control group, regular slow-wave and over-slow rhythm of Chinese medicine group had no obvious difference after treatment (P>0.05), but the Western medicine group still had obvious difference(2) In PDP analysis before and after the meal:Comparing with the control group, the PDP analysis before and after the meal decreased obviously in both Chinese medicine group and Western medicine group(P>0. 05), There was no obvious difference after treatment (P>0. 05).Experimental research:1. In model rats suffered from spleen deficiency syndrome, there was gastric emptying obstruction.2. In model rats suffered from spleen deficiency syndrome, the proportion of ICC cytoplasm in gastric muscular layer decreased, chondriosome swelled, endoplasmic reticulum dilated.3. In model rats suffered from spleen deficiency syndrome, mRNA and protein expression of c-kit gene in gastric muscular layer decreased .4. The XiangSha Six Mild-Drug Decoction could improve situation above.Conclusion1 There was gastric emptying obstruction in patients suffered from the spleen deficiency syndrome of chronic gastritis.2 In patients suffered from the spleen deficiency syndrome of chronic gastritis, electrogastro- movement decreasing was the mechanism of electrical physiology of gastric emptying obstruction.3 In patients suffered from the spleen deficiency syndrome of chronic gastritis, the XiangSha Six Mild-Drug Decoction could improve dyspepsia symptoms and gastric emptying obstruction, its mechanism had relationship with its improving electrogastro- movement.4 In model rats suffered from spleen deficiency syndrome, there was abnormality in the form and function of ICC in gastric muscular layer, The XiangSha Six Mild-Drug Decoction could improve situation above.