The Expression And Significance Of DD3 Gene In The Prostate Carcinoma

Objective: One of the theses was to to assess the clinical utility of diagnosis of DD3mRNA. The other goal is to explore the relation between prostate cancer and modulation of cell cycle. Methods: The expression of DD3 was analyzed by in situ hybridization in prostate specimens. We determined DD3 transcripts in urine sediments obtained after prostatic massage by real-time PCR. SiRNAs were transfected into LNCaP cells, Expression of DD3 mRNA was assayed by semi-quantitative RT-PCR. MTT method and clonogenecity experiment were employed to obtain parameters of proliferation of LNCaP cells. Cell cycle distribution was analyzed by flow cytometry. Results: The DD3 mRNA expression level has no statistical difference between normal prostate tissues and BPH. The DD3 mRNA expression level of prostate cancer has significant statistical differences. Both clinic stage and cell grade of prostate cancer are not statistically correlation to the expression level. All samples, except prostate cancer , were negative for the DD3 mRNA expression. DD3^PCA3-based RT-PCR assay of urine samples has a high sensitivity, specificity. Inhibition of DD3 expression resulted in G0-G1 phase arrest and inhibition of LNCaP proliferation. CONCLUSIONS: The expression of DD3 is very prostate specific. The quantitative RT-PCR assay for DD3 described bears great promise as a tool for molecular urine analysis. DD3 gene has a certain influence on promoting cell proliferation.