| Objective:the aim of our study was to investigate protective and therapeutic mechanism of Rehmannia Decotion in experimental AD rats.Methods:Male , Wistar 18 month Rat(n=120) were randomly divided into six groups.To induce AD,four groups rats received intraperitoneral injection of D-galactose combined with amyloid-β1-40 injection the bilateral Hippocampus.And the left, one goup served as blank controls ,the other as sham operated group.4 month rat (n=16) served as youth bank control, Rehmannia Decotion was administered by oral gavage to protective group before AD induction and therapeutic group after AD induction. Donepezil Hydrochloride Tablets served as control drug. Every indice was studied after 4 week AD induction.Result:In the behavior experiment ,model group rats' Escape latency was longest and number of times of pass safe platform was more than blank control group rats,white protective and therapeutic groups of Rehmannia Decotion rats were shorter and number of times pass safe platform was less than model group rats. By the light microscopy and electron microscopy observation,The neurocyte decreased and deformed in model group rats. Rehmannia Decotion can ameliorate this pathological changes. Measuring the activity of enzyme by chemical colorimetric method,we could detect that the activity of catalase, Glutathione peroxidase was lower in AD model group rats compared with blank control group rats , and these alterations were prevented by Rehmannia Decotion treatment.The activity of monoamine oxidase was higher in AD model group rats than blank control group rats , and this alterations were prevented by Rehmannia Decotion treatment.CMIAS image system was used for cover density,and PU value analysis.Detected by immunohistochemistry,the contents of Chat, nNOS and NMDAR2B protein in AD model group rats obviously decreased compared with blank control group rats. And these alterations were prevented by Rehmannia Decotion treatment.We used acid hybridization in situ in detection the expression of Tau mRNA , Tau mRNA in the Hippocampus in AD model group rats obviously increased compared with blank control group rats. And this alteration was prevented by Rehmannia Decotion treatment. Semiquantitative reverse transcription Polymerase chain reaction amplification of APP and glyceraldehyde3-phosphate dehydrogenase transcription performed. Densitometric analysis of PCR products showed that APP mRNA was significantly higher in model group rats than blank control group, protective and therapeutic groups of Rehmannia Decotion rats.APP mRNA levels were lower than model group rats,RT-PCR also was performed for SOD and ICE mRNA expression,significant difference was found in SOD and ICE amplification products between AD rats and blank control rats,Between protective and therapeutic groups of Rehmannia Decotion rats and model group rats. ICE mRNA expression in model group increased compared with blank control group.ICE mRNA expression in protective and therapeutic groups of Rehmannia Decotion rats decreased compared with Model group. SOD1 mRNA expression in model group decreased compared with blank control group.SOD1 mRNA expression in protective and therapeutic groups of Rehmannia Deco-tion rats increase compared with Model group.Conclusion.'These results suggest that AD rat model could be induced by intraperitoneral injection of D-galactose combined with amyloid-Pi.40 injection the Hippocampus.Rehmannia Decotion possesses a certain improvement of the ChAT,nNOS and NR2B content,decreased the MAO activity,consequently increased the learning memory level,and could decrease Tau mRNA expression. Rehmannia Decotion possesses a certain improvement of CAT and GSH-Px activity.Up-regulateing the SOD1 mRNA expression.According alleviating the damage of free radical,Rehmannia Decotion could downregulate Tau,ICE and APP mRNA expression, decrease the amount of senile plaques, NFT and Apoptosis in their brains. Kidney-essence weak, phelegm is the basic etiological factor for AD,And Replenishing kidney esse-...
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