| Objective:The aim of our study was to investigate protective and therapeutic mechanism of Rehmannia Decoction in experimental AD Model-rat.Methods:Male,Wistar, 18-month Rats(n=120) were randomly divided into six groups.To induce AD,four groups rats received intraperitoneral injection of D-galactose combined with amyloid-β1-40 injection the bilateral Hippocampus.And the left, one goup served as blank control group,the other as sham operated group.4-month rats (n=15) served as youth bkank control group, Rehmannia Decoction was administered by oral gavage to protective group before AD induction and therapeutic group after AD induction. Donepezil Hydrochloride Tablets served as control drug. Every indice was studied after 4 weeks AD induction. Through the behavior experiment,tests the change of study and memory of AD Model-rat. Measuring the activity of SOD and MDA by chemical colori-metric method. By the light microscopy and electron microscopy observes the change of morphology and structure in the hippocampus.By the method of immunohistoChem-istry detects the expression of C-FOS protein. By the method of RT-PCR detects the expression of MAO-B mRNA.Results:In the study of behavior experiment ,model group rats' Escape latency was prolonged and numbers of wrong were more than other group rats,while protective and therapeutic groups of Rehmannia Decoction rats were shorted and numbers of wrong were less than model group rats;In the memory of behavior experiment , model group rats' Escape latency was shorted and numbers of wrong were more than other group rats,while protective and therapeutic groups of Rehmannia Decotion ratswere longer and numbers of wrong were less than model group rats.Measuring the activity of enzyme by chemical colori-metric method,we could detect that the activity of SOD was lowest and MDA was highest in AD model group rats compared with other group rats,and these alterations were prevented by Rehmannia Decoction treatment. By the light microscopy and electron microscopy observation,the neurocyte decreased and deformed in model group rats. Rehmannia Decoction can ameliorate this pathological changes. CMIAS image system was used for cover density analysis.Detected by immunohistochemistry,the contents of C-FOS protein in AD model group rats obviously increased compared with other group rats. And these alterations were prevented by Rehmannia Decoction treatment.Densitometric analysis of PCR products showed that MAO-B mRNA was significantly highest in model group rats than other ogroup, protective and therapeutic groups of Rehmannia Decotion rats MAO-B mRNA level were lower than model group rats.Conclusion:Kidney-essence weak, phele-gm is the basic etiological factor for AD,and replenishing kidney-essence,Eliminating phlegm therapy is operative on treating AD;AD rat model could be induced by intraperitoneral injection of D-galactose combined with amyloid-β1-40 injection in the Hippocampus;Rehm-annia Decoction could improve the activity of SOD,decrease the content of MDA ,increase the learning memory level,better the pathologychange,decrease the expression of C-FOS, downregulate the expression of MAO-B mRNA.
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