Study On The Expression Of Novel Glioma Relative Genes

Objective: To analyze the glioma relative gene expressions states and find potential gene target used in the future by reverse dot-blot.Method: total RNA was extracted from gilomas and normal brain tissues, and reversely transcribed into cDNA. The probe was marked randomly and the dot blot was performed with the gene fragment which had been added onto nylon membrane. Using smart view image analysis system we contrasted the glioma development relative genes with β - actine, and made semiquantitation analysis by their ratios. Result: Contrast with the normal brain, there were 38 genes up-regulated and 24 genes down-regulated in grade two gliomas, 45 genes up-regulated and 17 genes down-regulated in grade three gliomas, 32 genes up-regulated -. 29 genes down-regulated and one unchanged in grade four glioblastomas. Grouped by pathology classification, Caspasel CDK4 c13 A20 EWS Caspase3 and EPS8 were significant in statistic. Compared maglinant gliomas with grade 2 gliomas, CDK4 MMP2 TOP1 c13 and RAD 17 were singificant in statistic. Conclusion: The expressions of genes in different tissues can be analysed semiquantitively by reverse dot blot. Using this way ,the overexpressions of two novel glioma relative genes (A20, EPS8) were found in glioma.Part II: Measure EPS8 A20 RAD17 expressions states in gliomasObjective: In the first part, we find three novel giloma relative genes (A20 EPS8 RAD 17) by reverse dot blot. Then, We will use RT-PCR to do more research about these genes expression states in different grade gliomas, which can confirm EPS8...