Study Of New Guided Bone Regeneration Collagen Membrane Combined With RhBMP-2 Utilizing Fibrin Gel As Carrier

To investigate the utility of fibrin gel as a carrier of rhBMP-2, BIO-GIDE? and another collagen membrane RuiNuo were used as a binding bearer which were covered by rhBMP-2 combined fibrin gel layer, for the sake of BIO-GIDE? and RuiNuo all have been applied in clinical use effectively and show good outcome for guided bone regeneration. In this research, the release of rhBMP-2 from combined collagen membrane and the cellular compatibility were detected in vitro. And the osteogenetic ability of these new guided bone regeneration membranes were inspected in vivo. So the application of these combined membranes in clinic was found in stronger foundation.In this study, we have three parts: 1, in vitro, human fibrinogen and thrombase were used to make the fibrin gel, and then rhBMP-2 was added in this fibrin gel. Smear this combined gel on the rough side of BIO-GIDE? and the inner side of RuiNuo. And the combined collagen membranes were carried out. By using the SDS-PAGE tech and UV spectrophotometer, the released rhBMP-2 was quantified, and the releasing behavior of combined collagen membrane was estimated. 2, cultured primary osteoblast cells of rat were seeded on the combined collagen membrane and SEM and MTT have been given to the cultured cell at different time point. And the cellular compatibility of these combined collagen membrane was estimated. 3, the critical sized defects of rat calvarium were made and the combined collagenmembranes were used as guided bone regeneration membrane with BIO-OSS? compared with titanium and uncombined collagen membranes as control. X-ray and faistoiogical and mmutnoMstochemisfric inspection were given to inspect the new bone under the membrane and the rhBMP-2 distribution.And then, we got such results as: 1, the methods of human fibrinogen and thrombase to make fibrin gel in lab are convenient. And the outcome of SDS-PAGE and UV spectrophotometer showed a rapid and small-dosage release of rhBMP-2 in die eariy stage when the combined membrane was tested in vitro. 2, the morphologic characters of rhBMP-2 combined fibrin gel were evaluated under SEM and showed an orange-like appearance. And such kind of appearance was then approved to be comfortable for osteoblast's attachment and proliferation m MTT. 3, fee immunohistochemistry outcomes showed that the releasing of rhBMP-2 was controlled by the fibrin and collagen membrane. Histoiogical results gave proof to osteogenetic ability of these combined collagen membranes by new bone quantity underneath the membrane in HE and Masson's tnchrome stain.The use of fibrin gel as rhBMP-2's carrier is feasible in lab. Moreover, a layer on the given side of collagen membrane can locate the release of rhBMP-2 for given area and occasion. Such kind of collagen membrane can accelerate the sew bone regeneration m viv?. So the technique to make fibrin gel and combine rhBMP-2 with collagen membrane may be considered for use in cine.