A Study Of The Relationship Between KAI1/CD82 Metastasis Suppressor Gene And Cervical Carcinoma

Objective:To research the expression of KAI1/CD82 metastasis suppressor gene in cervical carcinoma and study the effect of kail gene on the proliferation properties and invasive ability of Caski cervical cancer cell transfected by recombinant plasmid pCMV-KAI1 in vitro;also study the infection of HPV16E6, E7and HPV18 in cervical carcinoma to explore its effect on the expression of kail.Methods:Immunohistochemistry was used to detect the expression of KAI1/CD82 gene in formalin-fixed,paraffin-embedded specimens of 20 normal cervical epthelium,15 cervical in situ carcinoma,70 primary invasive cervical carcinoma as well as 29 lymph node metastases,Correlation of expression of KAI1 gene with clinicopathologic factors were statistically analyzed.The status of HPV16 E6, E7and HPV18 DNA was detected by PCR method; PCMV-KAI1 cDNA was transfected into highly malignant cervical carcinoma cell line CaSki by liposome,which had low levels of endogenous KAI1 expression .The expression of KAI1 protein was determined by immunohistochemistry,and the biological behaviors of the KAI1-transfected Caski cells were investigated by cell-matrix adhension and invasion assays.ResultS:l.KAIl/CD82 was expressed in both tumor and normal cervical epthelium, chiefly located in cell membrane,also locatecd in plasma in cancer cells; KAIl protein expression in tumor cells were down-regulation in invasive carcinoma and in situ carcinoma compared to controls(P<0.05).2.The protein expression of KAIl was not correlated with tumor FIGO stage> pathological subt)^^ depth of cervical infiltration^ lymphovascular space invasion .and the pelvic lymph node metastasis of cervical carcinoma(P>0.05),but its expression was significantly decreased in poorly differentiated tumours compared to that of well and moderately differentiated tumours,KAIl also expressed in lymph node metastases,but there have no statistically significant difference between them with expression in situ.3.The infection of HPV16 E6, E7and HPV18 in invasive carcinoma was not higher than in situ carcinoma , also not correlated with tumor clinical stage,histological grade^ depth of cervical infiltration lymphovascular space invasion and the pelvic lymph node metastasis,The expression of HPV16E6 was correlated with squamous cervical carcinoma,there was no correlation between expression of KAIl with infection of HPV16 E6, E7 or HPV18.4.The level of protein expression of Kail gene in Caski-kail cell after transfected by recombinant plasmid pCMV-KAIl was strengthed proved by Immunohistochemistry and RT-PCR(P<0.05).5. After transfected by recombinant plasmid pCMV-KAIl in vitro ,The proliferation ability of Caski cervical cancer cell were suppresssed compared with Caski and Caski-vector cell transfected by vector, and the migrative ability of passing through the membrane filte also decreased evidently(P<0.05).6.The ability of adhension of Caski-Kail cell to extracellular matrix was enhanced after trasfected with KAIl gene compared with Caski and Caski-vector cell,but there have no difference between Caski cell andCaski-vector cell (P>0.05) .Conclusion: The KAI1/CD82 metastasis suppressor gene might not a main factor in invasion and metastasis of cervical carcinoma , rough it affect the ability of proliferation and invasion of cervical cancer cell Caski in vitro.HPV infection doesn't affect the expression of KAI1 gene.