The Expression And Signficance Of ILK And PKB In Squamous Cell Carcinoma And Adenocarcinoma Of Lung

PerfaceLung cancer presens a major health problem in the world, more people died from lung cancer than the other cancers combined. Non - small cell lung cancers represent approximately 80 % of all lung carcinomas. moreover adenocarcinoma and squamous cell carcinoma are mores than other hostological subtypes. The mechanisms of lung carcinomas are not very clearly. Enormous efforts have been made to identify risk factors associated with the development of lung cancer and to explore potential preventive therapies.Intergin - linked kinase (ILK) is a recently identified protein serine/threo-nine kinase. ILK participates in regulating signal conduction via integrin and growth factors. ILK has been demonstrated to possess a number of oncogrnic properties. ILK activates protein kinase B (PKB) by phosphorylate it on ser 473. PKB is a major downstream target of ILK. Mounting evidence suggests that PKB perturbations play an important role in human malignancy. The ILK and PKB appears to play a prominent role in several processes thought to be critical in tumorogenesis, including aberrant cell proliferation, inhibition of programmed cell death, tedomerase activation and promotion of angiogenesis and tumor cell invasiveness.The aim of this research was to explore the roles of PKB and ILK in development and progression of lung carcinomas, by studying the expression and significance of PKB and ILK in squamous cell carcinoma and adecarcinoma oflung-MethodsImmunohistochemistry was applied to detection of ILK and p - PKB protein expression. Westers blot was introduced to detect PKB and p - PKB protein expression. Reverse transciptase polymerase chain reaction ( RT - PCR) was applied to detect ILK mRNA and PKB mRNA expression in lung carcinoma and correlated normal tissues. X2 test and Fisher s exact test were used to examine the association of p - PKB or ILK mRNA and protein expression.Restults1. Strong expression of ILK protein was detected in 74. 3% of adenocarcinoma and squamous cell carcinoma, whereas weak ILK expression was found in no - neoplastic epithelia and pulmonary alveoli. Strong expression of ILK was not correlated to age, sex, size of primary tumor, histological classification, dif-ferenentiation, lymph node metasis and P -TNM stage.2. There were significant different between the optional desities of p - PKB in tumor and normal lung tissue. There was obvious difference between the optional desities of p - PKB in morderate - well differention group and poor differ-entition group. No significant association was deteced between p - PKB protein expression and other clinical characterstics.3. The expression of PKB mRNA in squamous cell carcinima and adenocar-cina of lung was similial to the expression in the normal lung tissues. The expression of ILK mRNA in squamous cell carcinima and adenocarcinoma of lung was much higher than normal tissues. Both ILKmRNA and PKBmRNA were not dramatically associated to clinical characterstics.4. There were 45 samples expressing strongly ILK and p - PKB immunoac-tivity. There were 14 speciments expressing negnative stained with ILK and p -PKB immuhistochemically. The result of correlation analysis between p - PKB and ILK protein expression was dramarily significant.Conclusions1. The abnormal expression of ILK mRNA and protein is associated to the devolopement of lung carcinoma (squamous cell carcinoma and adenocarcino-ma) , the overexpression of which may be the potient biomarker for the carcino-genesis of lung carcinoma.2. The overexpression expression of p - PKB protein may participate in the development of lung carcinoma. Moreover it may be an important early event for tumor occurrence.3. Tere is significiantly association between the expression of p - PKB and ILK protein in the oncogensis of lung carcinoma.