| To investigate the effect of liposome mediated GGF2 gene transfer in vivo on neurological recovery of acute spinal cord injury in rats and its potential mechanisms. The contusion model of SCI in rats was established according to modified Allen's. The expression of GGF mRNA was measured through reverse transcription polymerase chain reaction in the rat spinal cord after SCI. The recombinant plasmids pEGFP-Nl-GGF2 and pIRES2-EGFP-GGF2 were successfully constructed and were injected into adult rats thoracic spinal cord with Transfectam liposome in order to investigate the expression of GGF2 and its effect on neurological recovery after SCI in rats. The results showed that the expression of GGF mRNA appeared to decrease after SCI, and the recombinant plasmids were successfully transferred into rats spinal cord and expressed GGF2, which promoted the neurological function recovery in rats with SCI. Conclusion: The early reduction of GGF expression in the injury site of spinal cord after SCI may be related to the widely necrosis or apoptosis of oligodendrocyte, and thus related to the difficulty of axon regeneration and remyelination in the spinal cord after SCI. Liposome mediated GGF2 gene transfer in spinal cord after SCI can promoted the neurological function recovery in rats with SCI. The mechanisms for GGF2 gene transfer in vivo on recovery of SCI in rats may be contributed as GGF2 promotes proliferation and survival of oligodendrocyte progenitors, prooligodendrocytes and oligodendrocytes in the spinal cord lesion after SCI, which enhances remyelination of the demyelination axon and promotes the regeneration of axon. |
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