The Effect Of DanHuangFang On The Acute Hepatic Failure And Liver Regeneration In Rats

Objective(1) To study the effect and mechanism of DanHuangFang on the acute hepatic failure and liver regeneration in rats.(2) To study the effect and mechanism of DanHuangFang on the liver after partial hepatectomy in rats.Methods:(1 ) 113 Wistar rats of SPF ,weighed230 - 250 g .were randomly divided into six groups: normal control group (8 rats), AHF group (25 rats), DHF group of small dose (20 rats), DHF group of middle dose (20 rats), DHF group of large dose (20 rats), pHGF group (20 rats). After being fasted overnight ( over 14h), on the second day of the experiment, all rats(except the normal group) were injected with TAA 600mgkg^-1 twice at same time for two days to induce the model of acute hepatic failure rat except the normal group injected with the 0.9 % salt solution hypodermicaily. From the first administration with TAA to the end of experiment, every rat was injected mixture solution contained 10% glucose 3ml, saline 1ml, and 20umol/L KCl·L^-1 every 8h to avoid hypoglycemia. The rats were treated with the 0.9% normal saline. DHF low, middle and high dosages, pHGF respectively every day for 3 days before TAA were injected. And observed the mortality of the rats in 48 hours, and the blood were collected to measure liver function (serum ALT, AST, TBil),tumor necrosis factor-a (TNF-α),after TAA were injected in the second time and the hepatic tissues were collected immediately. The pathological morphologies of hepatic tissues was observed under optics microscopy and the mitosis indexes of hepatic cells was measured. The expression of PCNA, signal regulatory protein-α (SIRP-α) and CD14 in liver cells was detected immunohistochemically , and the expression of CD14mRNA in liver was examined by reverse transcription polymerase chain reaction (RT-PCR).(2) 24 Wistar male rats .weighed 230 250 g .were randomly divided into four groups: normal control group (shame operated group), model group (heaptectomy group), DHF group, pHGF group, there was six rats in every group . Before operated every rat was fasted overnight for 6h and drinking water was prohibited for 6h.From the start to the end of the experiment, every rat was treated with the 0.9 % normal saline 4.0ml·kg^-1, DHF 10g·kg^-1 dosages, pHGF 1 ml/100 g respectively every day for 3 days before operating. The rats of shame operated group and heaptectomy group were injected 85% normal saline 1ml/100g. Operation was carried according to the method of Higgins and Panis. The rats of shame operate group were turned over left and middle leaves only. There was not one rat died during the operation. And after 48 hours,...