Studies On The Effects Of Gapsgf On The Expression Of Soluble Proteome And Nuclear Potein MDM-2 Of Mouse HepA Carcinoma

Objectives: Based on the results of our previous studies about the function and mechanism of anti-tumor of GAPSGF, this study focus on the effects of GAPSGF on the soluble proteome and nulear potein MDM-2 of mouse HepA carcinoma, to search new action targets of GAPSGF on the level of proteome, and provid more sufficient redical foundation for the clinic application of GAPSGF as anti-tumor agent.Methods : 36 mice of Kunming strain were divided randomly into three groups: control group, tumor group and GAPS group. The mice of tumor group and GAPS group were inoculated with HepA carcinoma. After that, all the mice were injected with normal saline and GAPSGF respectively for 10 days. After the last time of injection, all the mice were killed and were peeled off the tumor tissue. Then examined the expression of oncogene MDM-2 in liver and tumor tissue using immunohistochemistry, observeed the expression of soluble proteins with 1D-SDS-PAGE and 2-DE, and measured the expression of tumor suppression gene PTEN with dot blot and Western blot.Results: (1)In 1D-SDS-PAGE of soluble proteins, 16 bands were showed in control group,24 bands in tumor group and 18 bands in GAPS group. Compared with control group, 3 bands were down-regulated and 11 bands were up- regulated in tumor group. GAPSGF could make 3 down-regulated bands and 8 up-regulated bands be regulated back. (2)In 2-DE of soluble proteins, 101 spots were showed in tumor group and 85 spots in GAPS group. 54 spots only showed in tumor group and 38 spots only showed in GAPS group. There were 14 spots which showed both in tumor group and GAPS group but the quantity were different more than 2times.?The expression of antioncogene PTEN in tumor group was significantly lower than in GAPS group.@The expression of MDM-2 oncogene in GAPS group, was significantly lower than in tumor group (P<0.01) .Conclusions: ?The expression of soluble proteins in mouse HepA carcinoma is different from that in natural liver cell.?GAPSGF can effect the expression of soluble proteins in mouse HepA carcinoma.(3)GAPSGF can significantly enhance the expression of antioncogene PTEN, which is one of the soluble proteins in mouse HepA carcinoma, therefore GAPSGF can inhibit the growth of carcinoma.?GAPSGF can regulate down the expression of nulear protein MDM-2 oncogene in mouse HepA carcinoma.