The Expression And Its Significance Of Survivin, PTEN, VEGF In Hepatocellular Carcinoma

ObjectiveThis researh is in order to study the exprssion of Survivin, PTEN,VEGFin liver cell carcinoma and analyse its significance combined with theclinical pathological characteristics of patients with hepatocellularcarcinoma.Methodswe use immunohistochemical method to detected the expressin of thethree kinds of factor so that we can observe its expression andhistological localization in46cases of hepatic cell carcinoma and28cases of adjacent tissues. We use Western Blot method to testquantitative detection of protein. SPSS16.0software is used toanalyse the expression of the three kinds of factor and the relationshipbetween the three in hepatocellular carcinoma.Results1, The expression of Survivin in hepatocellular carcinoma tissue andhepatocellular carcinoma adjacent tissues are:71.7%,7.1%. Theexpression of PTEN in hepatocellular carcinoma tissue and hepatocellularcarcinoma adjacent tissues are:47.8%,92.9%. The expression ofVEGF in hepatocellular carcinoma and hepatocellular carcinoma adjacent tissues are:76%,17.9%. Western blot results show that therelative quantitative value of Survivin in hepatocellular carcinoma andadjacent group are:2.12±0.30,1.28±0.41. the relative quantitativevalue of PTEN in hepatocellular carcinoma and adjacent group are:1.13±0.25,3.11±0.40. the relative quantitative value of VEGF inhepatocellular carcinoma and adjacent group are:2.29±0.32,1.19±0.20.the two have differences by statistical analysis.2, The positive rate of Survivin expression in HCC is71.7%, and Theexpression of Survivin have relationship with the tumor diameter, thedegree of tumor differentiation and metastasis. The positive rate of PTENexpression in HCC is47.8%, and The expression of PTEN haverelationship with the degree of tumor differentiation and metastasis. Thepositive rate of VEGF expression in HCC is76%, and The expressionof VEGF have relationship with the tumor diameter, degree of tumordifferentiation and metastasis.3, the expression of Survivin is negatively correlated with the expressionof PTEN(r=-0.462), the expression of PTEN is negatively correlatedwith the expression of VEGF (r=-0.177), the expression of Survivin ispositively correlated with the expression of VEGF (r=0.247).Conclusion1, Survivin, PTEN and VEGF have relationship with the ocuurence ofhepatocellular carcinoma, the expression of Survivin and VEGF contributed to the ocuurence of hepatocellular carcinoma, the expressionof PTEN can induce hepatocellular carcinoma.2, the expression of Survivin and VEGF in HCC have closely relationshipwith hepatic cell carcinoma diameter, degree of tumor differentiation andmetastasis, it suggest that Survivin, VEGF promoted the development ofhepatocellular carcinoma. the expression of PTEN have closelyrelationship with the degree of tumor differentiation and metastasis,Theexpression of PTEN can inhibit the shift and deterioration of cancercell.3, the expression of Survivin can upregulate the expression of VEGF,the two are positively correlated. the expression of PTEN can inhibit theexpression of VEGF,the two have a negative correlation. the expressionbetween PTEN and Survivin against each other.