Ⅰ. The Peptides Binding To VEGF Receptors, KDR And FLT-1, Inhibit Angiogenesis And Tumor Growth Ⅱ. Biosynthesis Of Trichosanthins In Crown Tissue And Transformed Root Cultures Of Trichosanthed Kiriloii Maxim.

Neovascularization is critical for supporting the rapid growth of solid tumors beyond 2-3 mm in diameter and for tumor metastasis. Vascular endothelial growth factor (VEGF) is an angiogenic factor, and twp tyrosine kinase recptors have been identified for which VEGF acts as a high affinity ligand: an fins-like tyrosine kinase-1 (FLT-1) and a kinase domain receptor (KDR). VEGF binding to its tyrosine kinase receptors (KDR/FLK1, FLT-1) induces angipgenesis. Therefore, the interaction between VEGF and its receptors is the most appropriate to interrupt in order to inhibit angiogenesis. In search of the peptides blocking VEGF binding to its receptors, KDR and FLT-1, to inhibit tumor-angiogenesis and growth, a phage display peptide library had been screened with KDR and FLT-1 as target molecules, and some candidate peptides were isolatedIn this study, the DNA fragments coding the peptides K237/F56/F90, isolated from the phage display library, were cloned into a vector pQE42 respectively to express peptide fusion proteins DHFR-K237/F56/F90 in Ecoli M15. Enzyme-linked immunity absorbed (ELISA) analysis showed that DHFR-F56 and DHFR-F90 could bind to...