Study Of Glucocorticoid Receptor And Glucocorticoid Receptor MRNA In Peripheral Blood Mononuclear Cells From Patients With Myasthenia Gravis

Background: Myasthenia Gravis (MG) is an autoimmune disorder mainly mediated by acetylcholine receptor antibody (AChRAb). Glucocorticoids (GC) are potent immunosuppressive agents, and are clinically used in the treatment of MG and other autoimmune disorders. It is well known that giucocorticoid action is mediated through high affinity binding of steroid to the giucocorticoid receptor (GR). Clinically, individuals have different responsiveness to GC, and it takes different periods of time for them to get improved after administration of GC. Moreover, some patients are transiently impaired when treated with GC. A series of study about GR and GRmRNA in MG patients must be helpful for solving these problems.There have been a few reports about the study of GR in MG patients, yet none about GRmRNA.Material and Methods: Multi-point and single-point ~3H-Dexamethasone radioligand-binding assay and Scatchard analysis were used to measure the number of GR and dissociation constant (Kd) in PBMC from 20 healthy donors and 49 MG patients. GR numbers of PBMC before and after 48 hours of incubation in the...