| Lung cancer is a highly malignant tumor which is characterized by high incidence, high death rate and low 5-year surviving rate. This poor prognosis of lung cancer is closely related to the fact that most patients are not identified until the malignancy has reached an advanced stage. Therefore, new strategies are urgently needed to identify treatable, early stage of lung cancer. Recently, a method referred as SEREX (serological analysis of recombinant cDNA expression library) has shown powerful capacity to isolate tumor antigens recognized by humoral immune system, which also through detecting antibody responses to tumor antigens provides a novel form of serum marker for cancer detection. However, all SEREX studies previously reported just focused on cloning of antigens inducing IgG antibody response for cancer vaccine development. The role of these autoantibodies played in the cancer detection still remained elusive and little was known about cancer antigens recognized by autologous IgM antibody. Therefore, we modified the strategy of traditional SEREX in this study to isolate tumor antigens which elicited IgM antibody response. And autologous antibody spectrum against a panel of antigens identified in this study was further explored by a novel method of proteinchip established by ourselves, expecting to find out the relationship of these autoantibodies with the development of lung cancer and provide useful serum biomarkers for the detection and early detection of lung cancer.
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