Research Of Mechanism Of Antigen Recognition Of γδT Cells-Construction Of CDR3δ-chimeric Antibody And Its Antigen Recognition Specificity And Screening And Identification Of B Cell Epitopes Of SARS Cov Using Synthetic Overlapping Peptide Library

The present study included two principle issues as follows:1. Research of mechanism of antigen recognition of γδT cells-construction of CDR38-chimeric antibody and its antigen recognition specificity.γδT cells, the subset of T cells, are considered as the bridge of innate and adaptive immune that are involved in the infection immune, tumor immune and autoimmune. But up to new, a few ligands recognized by γδ T cell receptor (TCR) have been identified and the details of antigen recognition are not clear yet. The structural and functional characterization of αβTCR, γδTCR and antibody indicate that the specificities of antigen recognition of those three kinds of antigen receptors are all determined by their complementarity determining regions (CDRs). Moreover, γδTCR antigen recognition is non-major histocompatibility complex (MHC) restriction and more similar to antibody than αβTCR, and called "antibody like". Because of structural similarity of γδTCR and antibody, especially the similarity of the δ chain and heavy (H) chain in length and diversity of amino acids, it is reasonable to presume that the roles of δ chain and H chain are similar in the antigen recognition and CDR3δ might be the key determinant of γδTCR antigen recognition specificity.The former research in our lab has proved that synthetic OT3 peptides, a CDR38 fragment derived from y8 tumor infiltrating lymphocytes (TIL) of ovarian epithelial carcinoma (OEC) patients could bind not only OEC tumor tissues, OEC tumor cell lines...