The Expression And Modulation Of Maspin In Squamous Cell Carcinoma Of Larynx And Hypopharynx

Objective: Maspin inhibits metastasis of some cancer cells, and clinical studies have identified correlations between maspin loss and poor prognosis in several cancer types. Maspin was found to be significantly low expressed in some cancer samples as compared with matched normal tissues. However, the expression and regulatory mechanism of maspin in squamous cell carcinoma of larynx and hypopharynx remains unclear. The purpose of this study was to investigate the expression of maspin in squamous cell carcinoma of larynx and hypopharynx as well as it's relationship with clinicopathological features, such as age, gender, cell differentiation, staging, diseased region, metastasis and prognosis factor. Microvessel density(MVD) and Lymph vessel density(LVD) was respectively labeled using CD105 and LYVE-1 antibody. The contribution of maspin to the formation of blood vessel and lymph vessel was studied with clinicopathological features. We investigated the expression of p53 and it's role in regulation of maspin expression.Methods: A total of 78 cases of laryngeal squamous cell carcinoma(LSCC) and 53 cases of hypopharygeal squamous cell carcinoma(HPSCC) who underwent operation at Qilu hospital of Shandong university between Sep. 1998 and Sep. 2000 were investigated. All patients were not underwent radiotherapy or chemotherapy. They were follow up for 36 months to 83 months, but only 41 cases of LSCC and 28 cases of HPSCC were obtained integrity information. 30 cases of adult laryngeal papilloma, 30 cases of squamous atypical hyperplasia and 30 cases of metastatic...