A Long-term Investigations Of Changes Of Patho-morphism,Neurochemistry And Neurological Deficit After Ligation Both The Posterior 1/3 SSS And Bridging Veins In Rabbits

Part 1Modeling of An Acute Experimental Cerebral VenousInfarction in RabbitsObjective: To establish a stable model of an acute experimental cerebral venous hemorrhagic infarction in Rabbits. Methods: 35 rabbits were randomly divided into 3 groups. Group A, the operation group in which the posterior 1/3 superior sagittal sinus (SSS) was ligated from rostral to caudaul(n= 15); Group B, the operation group in which both SSS and bridging veins were ligated(n=15) and group C was the sham operation group(n=5). Water content ratios of the brain(%) and the range of cerebral venous hemorrhagic infarction were obtained at an interval of 8h,24h,48h, respectively(n=5 for each sub-group) after operation. Macroscopic examination, lightscopy and electron-microscopy were compared with these data. Results: The contents of water in brain tissue of group A at 8h and group B at 8h,24h,48h after operation were higher than those of group C(P<0.01). The range of cerebral venous hemorrhagic infarction of group B at 24h,48h after operation was remarkably wider than those of group A(P<0.01). The cortical lesions were stained with Evans blue dye after 24h in group B. Histologically, in all cases signs of acute venous hemorrhagic infarction could be demonstrated in every case in group B at 241k 48h after operation, even though there were only 5 of 15 rabbits in group A. Conclusion: Modeling of ligation of both the posterior 1/3 SSS and bridging veins to establish a stable model of an acute experimental cerebral venous hemorrhagic infarction in Rabbits is feasible.