Experimental And Clinical Preliminary Research On Laser-induced Fluorescence In The Diagnosis Of Colorectal Carcinoma

Objective: To analyze the characteristics of laser-induced autofluorescence (LIAF) of normal colorectal tissue and colorectal carcinoma tissue and establish the criterion of laser-induced autofluorescence (LIAF) spectroscopy in the differentiation of normal colorectal tissue and colorectal carcinoma tissue, and then study the feasibility of LIF spectroscopy in the diagnosis of colorectal carcinoma.Methods: 56 surgical specimens of normal colorectal tissue and colorectal carcinoma tissue were collected. The LIF spectra were examined by a detecting system which consists of a YAG laser (wavelength 370nm) and an optical multichannel analyzer (OMA). 120 spectra of 42 surgical specimens were detected, including 57 spectra from normal colorectal tissue and 63 spectra from colorectal carcinoma tissue. Spectroscopic differences between normal tissue and cancerous tissue have been found which could be used as criterions to distinguish between them. Every tested spot was marked and the pathological examinations were performed in order to compare with the criterion.Results: 1.The location of the principal spectral peaks of normal...