The Construction Of Two Dimensional Electrophoresis Method And Analysis Of Protein Expression In Mouse Myeloid DC

Being the most potent professional antigen presenting cells, the Dendritic cells (DC) is endowed with the unique ability to stimulate naive T cells to initiate primary immune responses. In addition, the DC plays a critical role in the maintenance of self-tolerance by curtailing T cell responses directly or indirectly through the generation of CTL and CD4+Th regulatory cells. Many researchers have made emphasis on it in immunology and other related course.The myeloid DC has different function during their different differentiation and development stage. Mediated by adhesion molecule, the DC enters inflammation tissue with the potent ability of antigen endocytosis and treatment, but it is endowed with the weak ability to stimulate naive T cells. It is an important topic for tumor biology therapy to enhance the body special immune ability to anti cancer by researched on DC. And it has afforded good clinical results that the DC is made as tumor vaccines for gene therapy at home and abroad. If we make the immature DC into mature DC by introducing antigens, the vaccines will be better at clinical application.The negative-strand RNA viral vector system based on the Sendai virus (SeV) is able to efficiently infect many animal cells and more safer than other vectors because it can not change the chromosomes in the nucleus in theory. People like it as the vector for gene therapy, gene vaccine, analysis of gene function and protein production.