| Indigowoad root is one of a few well-recognized anti-virus traditional herbs at present time. It has been shown that some specific lipid-solvent fractions such as inderubin can be extracted more effectively by ethanol than by water.Indigowoad root agent is obtained by alcohol reflux methed, extra-filtration that can filter out 10,000 or above MW of substances in order to decrease their cytotoxincity and finally prepared into 10% solution, which is shallow-yellow fluid. The role that this homemade agent play in anti-HSV1, anti-Sendai has been explored not only to supplement its anti-virus activities but also to provide theoretic background underlying its anti-virus mechanism.CPE and MTT method are employed to illustrate objectively and sensitively both the extent to which HSV can cause pathgencity on Vero cell and the prevention from the virus attack in order to show the anti-HSV effect. The result has shown solution of such titer as 1:2-1:8 has therapeutic and direct deactive, whereas less preventive, effect on HSV.RBC absorption assay has verified the similar result on Sendai virus using the same titer of Indigowoad root solution.These outcomes imply that the agent's anti-virus mechanism lies in not induce of host preventive function against the virus, but direct kill effect, prevention of absorption to host cell or inhibition of the virus replication. However, the exact stage of virus replication process on which the herb extract has influence has to be further studied.
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