Effect Of HTFF2 Gene Therapy On The Healing Of Experimental Gastric Ulcer In Rats

BACKGROUNDGastric ulcer is clinically prevalent disease with repeated courses and high recurrence rates. At present, in the curing of gastric ulceration, there is still some gastric ulcer which can not be cured under the conventional treatment of restraining acid secretion, protecting gastric mucosa and resisting HP. In addition to ulcer itself, infection of HP, smoking, psychology and so on, the deficiency or decrease of some growth factors (GF) has become one of the reasons for the refractory ulcer. Trefoil factor family (TFF) peptides are a group of factors protecting the gastrointestinal mucosa. TFF2 is the only member of the trefoil factor family (TFF) peptides which bearing two trefoil domains (P-domain) and glycosylated micromolecule protein. And it is closely distributed in antral glands (GA) and duodenum where peptic ulcer most likely occurs. Normally, trefoil family factor 2 (TFF2) is mainly synthesized and secreted by mucin neck epithelial cells in the body mucosa and antral glands (GA). The secretion of TFF2 is increased after mucosal injury. It is expressed specifically in peptic ulcer, implying it plays a key role in keeping the complement of gastrointestinal tract mucosa and promoting rapid reparative process. Recent researches indicate that hTFF2 is respectively related to peptic ulcer. Abundant experiments in vitro and in vivo have demonstrated that hTFF2 can protect the epithelium in gastrointestinal tract and promote the healing of damaged mucosa. TFF2 can reduce H~+ infiltration and mucosal injury from noxious substance, stimulate the migration of epithelium, participate the restitution of gastric mucosa epithelium after injury. Nevertheless hTFF2 protein can not be obtained easily and is every expensive etc, so we try to treat the experimental gastric ulcer in rats by synthesizing TFF2 gene through gene slicing and using naked DNA which encoding hTFF2.AIMSBased on the healing of acetic acid-induced rat gastric ulcer, the study was aimed to: 1. determine whether the right fragment of hTFF2 can be obtained by gene slicing; 2. whether a single local injection of naked plasmid encoding hTFF2 can promote the healing of acetic acid-induced gastric ulcer in rats; 3. evaluate the effects...