The Study Of Apoptosis Induced By P21～(WAF/CIPP1) Gene Transfection Into Renal Carcinoma Cells

Renal carcinoma is a common malignant tumor in Urology. Exploring the mechanism of renal carcinoma and studying the factors that affect the biological behaviors of renal carcinoma are of great significance. Apoptosis is closely related to tumors and has become the hot spot of study all over the world. However, the molecular mechanisms of apoptosis are not clear. In recent years it was found that apoptosis caused by p53 gene usually followed by the expression of P²¹ protein. It suggest that p21^WAF1 gene play an important role in apoptosis. Therefore it was very important to investigate the relationship between p21^WAF1 gene and apoplosis and to explore the molecular mechanisms of apoptosis .The main contents and results of this study were as following.1. Using the method of immunohistochemistry, we found that the expression of P²¹ protein in GRC-1 cells was negative.2. The influence of p21^WAF1 gene transfection on apoptosis of GRC-1 cells.Using the lipofection-mediated transfection method human p21^WAF1 gene in the retrovirus PCEP- p21^WAF1 vector was transferred into GRC-1 cell line, screened by bygromycin. Success of transfection was confirmed by in situ hybridization, Dot blot and immunohistochemistry. Apoptotic cells induced by transient transfection of p21^WAF1 in GRC-1 cells were observed under trans-electron microscope. Apoptotic cells induced by stable transfection of...