14-3-3? Regulates Proliferation, Apoptosis, Invasion And Metastasis In The Renal Carcinoma Cell

Objective: Renal carcinoma is one of the most common tumor of urogenital system,and clear cell carcinoma is the major pathological type.It has been reported that the expression of 14-3-3? gene in renal carcinoma tissue is significantly lower than that in the normal renal tissue.At present,the treatment of the renal carcinoma still mainly depends on surgical intervention.The expression of 14-3-3? gene in the renal carcinoma tissue is higher than in the normal kidney tissue,that might imply the potential of14-3-3? gene in the management of renal carcinoma.The function of the 14-3-3? gene in the renal carcinoma cells was studied in order to clarify whether it could become a gene target in the treatment of renal cell carcinoma.Methods: The endogenous expressions of the 14-3-3? in the renal carcinoma cell lines including 769-P,786-O and ACHN were detected by the method of RT-PCR and Western blot.Then,14-3-3? si RNA was transfected with lipo3000 into the cell lines,and the transfection efficiency of 14-3-3? si RNA in the 769-P and ACHN cells was determined by the method of RT-PCR and Western blot.In addition,the functions of the transfected cells were investigated,including cell invasion and metastasis,cell proliferation,cell cycle and apoptosis.Result: The 14-3-3? m RNA level was highest in the 769-P cells,and it was less in ACHN cells and lowest in the 786-O cells.Similarly,the 14-3-3? protein level was highest in the 769-P cells,and it was less in ACHN cells and lowest in the 786-O cells.Then,14-3-3? si RNA was transfected with lipo3000 into the three cell lines.The 14-3-3?m RNA level was significantly lower in the transfected 769-P and ACHN cells than that of the untransfected ones.In addition,14-3-3? protein level was also significantly lower in the transfected 769-P and ACHN cells than that of the untransfected ones.The capacity of cell invasion and metastasis were increased in the 769-P and ACHN transfected with14-3-3? si RNA than those of the control groups,and the percentage of cells arrested in G1 stage was also higher in the 769-P and ACHN cells transfected with 14-3-3? si RNA than that of the control groups,that suggested that the 14-3-3? gene could contribute to the cell cycle arrested in the G1.Moreover,the rate of cell apoptosis in the control groupwas three times of that of the 769-P cell transfected with 14-3-3? si RNA,and the rate of cell apoptosis in the control group was two times of that of the ACHN cell transfected with 14-3-3? si RNA.The capacity of cell proliferation was also increased in the 769-P and ACHN cells transfected with 14-3-3? si RNA than that of the control group.Conclusion: The expression of 14-3-3? gene can be detected in renal cell carcinoma.The capacity of cell invasion,metastasis and proliferation in renal cell carcinoma could be suppressed by 14-3-3? gene.The cell apoptosis in renal cell carcinoma could be promoted by 14-3-3? gene.Additionally,the 14-3-3? gene could contribute to the cell cycle arrested in the G1.