Construction Of Cyclin D1 Antisense RNA Eukaryotic Expression Vector And Its Effect On The Malignant And Multidrug-resistant Phenotype Of Gastric Cancer

Uncontrolled, unlimited and accelerated multiplication is one of the most fundamental biologic behavior of cancer cells. The result of the malignant proliferation is a block in the ability of cancer cells to undergo quiet, terminal differentiation and / or apoptosis. The control mechanism of cell cycle is now believed to be related to cellular growth, temporary arrest, differentiation and / or apoptosis. Studies on the function of oncogenes and tumor suppressor genes indicate that most of these genes are can influence this mechanism in differetn control levels of cell cycle regulatory net. This has led to the realization that the key control protein in cell cycle regulatory net can also be directly invoved in oncogenesis. So, further research on the key cell cycle control protein will provide a better understanding of cell proliferation and contribute to the solution of some critical life science problem such as cellular differentiation, senilty and carcinogenesis.The current advances in molecular biology have established a model of cell cycle regulatory net which the core is cyclinæ¡ŸDK holoenzymes. Cyclins are regulatory subunits of the holoenzymes. The activation and cell cycle phase specificity of the holoenzymes depend on cyclins. Cyclind D1 is a key controller of G1 phase progression in most...