Effects Of L-Nitroarginine, L-Arginine And Aminoguanidine On The Recovery Of Traumatic Facial Paralysis And Their Cytologic Mechanism In Rat

Maxillofacial impact injury is a multiple, harmful wound with many complications, and facial nerve contusion and then facial paralysis is one of complications. As the spontaneous recovery of traumatic facial paralysis is often difficult, it becomes a difficult point of clinical treatment. To thorough study the cytologic basis and the molecular mechanism on the recovery of traumatic facial paralysis has become the key to recognize and solve the problem of traumatic facial paralysis eventually. Our study firstly established the experimental animal model of traumatic facial paralysis in rats, and then evaluated the effects of constitutive NOS inhibitor: L-nitroarginine, inducible NOS inhibitor: aminoguanidine and NO precursor: L-arginine to intervene the recovery of traumatic facial paralysis and the corresponding changes in cNOS, iNOS, c-fos, IL-6 and OX42 immunoreactivity (ir) in facial nucleus and immunohistochemical histological changes in facial nerve and surrounding tissues. It was found that the animal model of traumatic facial paralysis made by multiple function impact system for trauma was reproducible, easy to be made, accurate of measuring velocity, controllable of impact force and could cause different degrees of facial paralysis ,and it was an ideal model for study the traumatic facial paralysis. Under the condition of 10m/s impact velocity, 0.5cm of displacement, complete facial paralysis was made and the facial nerve was not broken. So, it became the standard impact condition and was used in next experiments. In intervention experiments, L-nitroarginine could remarkable inhibit the recovery of traumatic facial paralysis, L-...