A Study Of The Clinical Significance And The Mechanism Of Hepatocellular Carcinoma (HCC) Of Hepatitis B Virus Basic Core Promoter (HBV BCP) Mutation In Patents With Chronic Hepatitis B Virus Infection | Posted on:2007-11-15 | Degree:Doctor | Type:Dissertation | Country:China | Candidate:L Y Huang | Full Text:PDF | GTID:1104360185952175 | Subject:Oncology | Abstract/Summary: | PDF Full Text Request | [ Objective ] In order to investigate the clinical significance and the mechanism of hepatocellular carcinoma (HCC) of hepatitis B virus basic core promoter (HBV BCP) mutation in patients with chronic hepatitis B virus infection. [Methods] (1) project subject: 176 patients (Chronic hepatitis B with mild , moderate and sever; liver cirrhosis, Chronic fulminant hepatitis and HCC ) with chronic hepatitis B virus infection were studied. (2) project methods: ①The A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1764 were determined by the method of polymerase chain reaction (PCR) microplate hybridization ELISA in these patients. ② The serum cytokines (IL-10, IL-12, TNF-α and IFN-γ) of these patients were measured by specific-ELISA. ③ Serum HBV DNA of these patients were detected by fluorescence PCR. ④ Serum HBV markers were determined by ELISA.⑤ The...
| Keywords/Search Tags: | Hepatitis B virus, Polymerase chain reaction, basic core promoter, gene mutation, interleukin 10, interleukin 12, tumor necrosis factorα, interferonγ | PDF Full Text Request | Related items |
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