Studies On Artificial Culture And Factors Influencing Production Of Some Active Constituents In Grifola Umbellata And Armillaria Mellea

The solid cultural character and submerged cultural conditions to Grifola umbellata, companion fungus andArmillaria mellea, factors influencing formation in sclerotia of G umbellata and formation of fruitbody in A mellea, and factors influencing formation of polyporusterones, polysaccharides in G umbeUata and melanin in A. mellea were systematically studied in this thesis.The nutritional requirement is simple for the growth of G umbellata which can grow in media only have carbon source and nitrogen source. Malt-extract agar(MEA), GPC agar and GPY agar were suitable for solid culture to G umbellata. Better adaptability to carbon sources and organic nitrogen source except for urea were observed during the culture of G umbellata, the results showed that the aerial hypha morphous and mycelial growth velocity were different when this funus grew in media with different carbon sources. As growth factors, corn steep liquor, yeast extract, milk powder, thiamin, extract of companion fungi and A mellea mycelia, activated clay, diatomite and kaolin could promote growth of G umbellata. Optimal growth temperature for G umbellata was 25℃.Three suitable media for the growth of companion fungus were malt-extract agar, GPC agar and GPY agar. Better adaptability to carbon sources and organic nitrogen source were observed during the culture of companion fungi. Suitable temperature for the growth of G umbellata was 25℃.Companion fungus was grew best in carrot-dextrose agar but which was not for G umbellata.The optimized medium for obtaining high mycela yield of G umbellata was soluble amylum 20 g·L^-1, bean cake extract 200ml ·L^-1, corn steep liquor 20 ml·L^-1, thiamine 0.25 g·L^-1, pH 6.5. The optimized medium for obtaining high mycela yield of companion fungus was soluble amylum 20 g·L^-1,bean cake extract 200 ml ·L^-1, corn steep liquor 20 ml ·L^-1, ammonium sulfate 1 g·L^-1, magnesium sulfate 1 g·L^-1, calcium chloride 2 g·L^-1, pH 6.0.Pilot scale production for G umbellata mycelia in submerged culture was experimented in a 3m³ stirred-tank bioreactor. Inoculums was prepared in the corn meal liquid medium in shake flasks or malt-extract liquid medium in stationary culture flasks. The size of inoculums was about 1% of the first class seeding bioreactor working volume (300L). Seeding culture was carried out at 25 ℃ and aeration rate of 1 : 0.5 for 190h, which mycelial dry weight achieved 0.23%. Working volume of...