Study On The Mechanisms Of The Rhizomorph Of Armillaria Mellea In Anti-aging Of Caenorhabditis Elegans

Aging is characterized as widespread and intricate process of a physiological phenomenon in vivo, which is influenced by both environmental and genetic factors. With the development of science and technology, the molecular mechanism of the aging process has been mastered and the study on slow aging drugs has been seen as the current one of the popular scientific research.According to the oxidative damage theory, which is seen as more influential in many aging theories, the accumulation of free radical is one of the major factors in the aging process. The free radicals which are generated by aerobic metabolic processes of organisms can be cleared by antioxidant enzymes. When the body is in an unfavorable environment, the free radicals that can not be cleared by antioxidant enzymes will cause some damage to the body, such as destruction of cell membranes, DNA damage. The damage which can not be repaired led to the produce of the body of disease and aging. Therefore, to strengthen the organism free radical scavenging ability and the aging process can be delayed.We use Caenorhabditis elegans as a model organism to study the mechanisms of the rhizomorph of Armillaria mellea in anti-aging, the main contents of the study were as follows:Screening the best concentration of AMP:Under normal culture conditions, synchronization of Caenorhabditis elegans were treated in medium containing AMP. After a certain period of time, the survival rate of Caenorhabditis elegans was observed under heat shock treatment. The results showed that the rhizomorph of Armillaria mellea (two components:AMP-1, AMP-2) can improve the survival rate of Caenorhabditis elegans and the efficacy of AMP-2is superior to the AMP-1.AMP to Caenorhabditis elegans protection experiments:the Caenorhabditis elegans were treated in medium containing AMP under normal conditions and the Caenorhabditis elegans longevity and fecundity were observed. The results showed that the fecundity of the Caenorhabditis elegans is the same as before but the life span of Caenorhabditis elegans were extended. APM on the protective role of Caenorhabditis elegans:The mutants of Caenorhabditis elegans were treated in medium containing AMP under normal conditions for some time, then transferred to incubator under oxidative stress. The expression of HSP-16.2::GFP in CL2070and SOD-3::GFP in CF1553were investigated by fluorescent microscopy. The results showed that the expression of HSP-16.2and SOD-3was enhanced in Caenorhabditis elegans.AMP’s role in the repair of the Caenorhabditis elegans:The mutants of Caenorhabditis elegans which has been oxidative damage were treated in medium containing AMP under normal conditions for some time. The expression of HSP-16.2::GFP in CL2070and SOD-3::GFP in CF1553were investigated by fluorescent microscopy. The results showed that the expression of HSP-16.2and SOD-3was reduced in Caenorhabditis elegans.Further study on the mechanisms of AMP in anti-aging of Caenorhabditis elegans:The mutant TJ356(DAF-16::GFP) were treated in medium containing AMP under normal culture conditions, then observed subcellular DAF-16localisation. The results showed that the DAF-16protein to the cell nucleus transfer rate was increased.These results suggest that AMP can delay the aging of Caenorhabditis elegans and the protection mechanism is closely related with AMP antioxidant capacity.