Research Of The Effect And Mechanism Of Platelet-rich Plasma In The Repair Of Goat Skull Defects

Growth factors released from activated platelets initiate and modulate wound healing in both soft and hard tissues. A recent strategy to promote the wound-healing cascade is to prepare an autologous platelet concentrate suspended in plasma, also known as platelet-rich plasma, that contains growth factors and administer it to wound sites.Purpose : To study the effects of platelet-rich plasma (PRP) on the proliferation of the marrow mesenchymal stem cells (MSCs) cultured in vitro to elucidate the cellular and molecular mechanism by which PRP accelerates bone repair,, and to study the effect of platelet-rich plasma in the repair of goat skull defects.Methods: 1. The goat MSCs were cultured in vitro and randomly divided into the experimental group and control group. In the experimental group, the M SCs were interfused with PRP (10 μ l/ml culture media). The proliferation ability of the cells was tested by growth curve and MTT; Platelet-derived growth factor-BB and transforming growth factor- β₁ were measured in the human platelet-rich plasma using the enzyme-linked immunosorbent assay method. 2. The 16 adult goats were randomly devided into 4 groups. A diameter 2.2cm calvaria defect was created in each goat. Coral/PRP mixture were impanted in the skull defects, and coral granules acted as control; Autologous bone/PRP mixture were implanted in the skull defects and autologous bone granules implanted alone as control. Bone regeneration was assessed by histological analysis and biomechanical test in 16 weeks .Results: 1. PRP could stimulate the MSCs proliferation. The growth curve and MTT value showed that MSCs proliferated more in the experimental group than in the control group( P< 0. 05). 2. An 4-fold increase in platelet concentration was found in the goats' platelet-rich plasma compared with that of whole blood (baseline whole blood, (3.52 ± 1.09) × 10¹1platelets/L; PRP, (14.05±3.58)X 1011 platelets /L). On average for the whole human blood as compared with autologous platelet-rich plasma, the platelet-derived growth factor-BB...