| PurposeWhen Marx led the PRP technique to the stomatology domain for the first time, itcausd the galactic intrest and passions for the overseas dentistry group. At the sametime, it offered new ideas about the recovery therapy of the tissue defects and traumasin the following domains such as the oral planting, peridental remodeling and the jawbones remodeling, etc. It has excellent developmental perspective in the stomatologyclinical application. With the gradually raise of the cognition with the PRP technique,and the extent of the PRP application magnified, the demand for the PRP in clinic willincrease, too. While the abstraction and preparation in temporary were already far tosatisfy the clinical demand, but how to store is the only way to solve the problem. Atpresent, the study about the PRP preservation mainly concentrate on the plateletmorphology and the vitro aggregation functional parameter post-conservation, and thevariation about the blooding clotting and stopping after infusion in clinic, and so on.The PRP techniques used in the department of stomatology are mainly about how toultilize the protein GF of high density and to encourage the tissue to repair and rebuild.The study about the estimation of the platelet in the appearance, metabolism, functionand trauma and the contents variation of the GF in promoting tissue reparativeregeneration have not been reported yet. Whether the PRP has the ability to enhancetissues reparative regeneration or not need further studies. The objective of this essay isto invest the blood platelets count(BPC), WBC density, RBC density, mean platelet volume (MPV), PH, oxygen partial pressure(PO2), carbon dioxide partialpressure(PCO2), lactate dehydrogenase (LDH) density, lactic acid (LAC)density aboutthe PRP pre and post conservation with mild and successive agitating in 20～24℃, andto compare the contents variation of the PDGF-AB and the TGF-β1 in PRP under theaviated state from the point of stomatology clinical medicine, then to estimate theappearance, metabolism, function and trauma of the platelet in the PRP postconservation with mild and successive agitating in 20～24℃, to investigate thecapability changes in promoting tissue reparative regeneration, to provide theexperimental base for clinic about efficient conservation, reasonable utilization, then tomake sure the clinical therapeutic effect.Materials and MethodsChoose the health people conformed to the People's Republic of China todedicate blood the physical examination standard, ACD-A anticoagulation venousblood 200ml, use the albuginea replasm method to prepare PRP, chooses 20～24℃continuously temperate agitating preservation condition, invest the blood plateletscount(BPC), WBC density, mean platelet volume (MPV), PH, oxygen partialpressure(PO2), carbon dioxide partial pressure(PCO2), lactate dehydrogenase (LDH)density, lactic acid density about the PRP pre and post conservation with agitating mildand successive in 20～24℃. Before test the concentration of the PDGF-AB and theTGF-β1, reference the PRP application in clinic, utilize the cattle fibrin ferment with10％CaCl2 liquor to activate the specimen, activate the platelet to release GF into bloodserum so the quantitative analysis can be done. Test the contents of PDGF-AB and theTGF-β1 with double antibody sandwich ABC-ELISA. Analysis and treat the data withthe SPSS 11.5.ResultsThe measured index result: the PLT number(×109)/L of the VB plasma, FPRP,SPRP are: 1604±29.09, 8804±279.96, 8504±296.89; MPV(fL)are: 8.564±0.54, 8.244±0.80,9.924±0.48; WBC(×109)/L are: 6.54±1.19, 2.64±1.99, 2.54±1.91; RBC(×1012)/L are: 4.6±0.76, 0.028±0.023, 0.025±0.02; PH are 7.364±0.03, 7.093±0.066, 7.034±0.078;PO2(mmHg)are: 39.72±9.23, 103.63±13.94, 113.89±7.69; PCO2(mmHg)are:45.50±6.12, 58.95±6.85, 32.7±4.92; LDH(mmol/L)are: 180.83±32.52, 231.87±36.85,352.53±61.22; LAC(mmol/L)are: 1.40±0.23, 1.58±0.28, 30.18±5.1. The PDGF-ABconcentration(pg/ml) of the VB plasma, FPRP, SPRP are: 1.69±0.36, 56.12±11.72,58.17±12.1; the TGF-β1 concentration(pg/ml)are: 56.19±14.55, 331.12±64.94,365.55±73.13. The calculated index results: the PLT total number in VB(×1010):3.7±0.66/per bag, the PLT total number in FPRP(×1010): 2.6±0.6/u; FPRP WBC totalnumber(×107): 7.3±4.93/u; FPRP RBC total number(×108): 8.0±5.73/u(the PRPproduced in 200ml VB blood plasma is one u); the PEF of the FPRP: 5.3±1.0; PRR(％):69.12±8.6; the PDEF of the FPRP: 36.94±12.44; TEF of the FPRP: 6.59±2.03;PDSR(％) of the SPRP: 104.17±11.28; TSR(％)of the SFPRP: 110.68±8.29.Comparethe SPRP group with the FPRP group: BPC have no significiant difference(P＞0.05);the WBC concentration, the RBC concentration, MPV, pH, PO2, PCO2, LDH, LAChave significant difference(P＜0.05); PDGF-AB content have no significantdifference(P＞0.05); the TGF-β1 content have significant difference(P＜0.05), theconcentration increased 10％post conservation compared with pre-conservation.Conclusion1. The method of the PRP conservation with agitating mild and successive in 20～24℃is easy to obtain and requests few equipment, and operate easy in clinic, so it isthe the first choice in few neccecity and short-term store about the PRP in stomotologydepartment.2. The PRP before and post conservation with agitating mild and successive in20～24℃around three days, the blood platelet quantity not show obvious change,while the physico-chemical index show that the shape and metabolism of the bloodplatelet have changed in some extend.3. The PRP before and post conservation with agitating mild and successive in20～24℃around three days, the content of PDGF-AB not obvious change under state of activation, the content of TGF-β1 increases about 10.68％under the state ofactivation. 20～24℃continuously temperate vibrations preserves three days has notcreated PDGF-AB and TGF-β1 content reduces.
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