Alteration Of Several Molecular Biological Markers In The Development And Progression Of Lung Cancer

Objective Chromosome 3p and 9p frequently exhibit genetic materials loss in lung cancer tissues, 3p14.2, and 9p21 are the major loss region. To study the microsatellite alterations (at 3p14.2 and 9p21) and the alteration of FHIT and p16 gene and Ki-ras gene.Methods 49 lung cancer and 16 metastatic hilar node tissues RNAs were examined for expression and mutation of p 16 gene exon2-3 fragment by RT-PCR and SSCP. 34 lung tissues away from lung cancer, 47 lung cancer and 16 metastatic hilar node RNAs were examined for expression and mutation of FHIT gene by RT-PCR and SSCP. 38 lung cancer genomic DNAs were examined for microsatellite alteration at 3pl4.2 and 9p21 regions, and for mutation of Ki-ras.Results and Conclusions â‘  No FHIT gene abnormality occurred in normal lung tissues, 20% no-neoplastic lung tissues exhibited loss of FHIT gene exon5-9 transcripts. â‘¡ 3p14.2 LOH is the common event in lung cancer (81.6%). There is no statistically correlation between 3p 14.2 LOH and sex, smoking history, TNM stage, and differentiation degree etc. â‘¢ loss of FHIT gene transcripts is frequent event in lung cancer tissues (68%) and matched metastatic hilar nodes (93.7%). There is no statistically correlation between loss of FHIT gene transcripts and sex, smoking history, TNM stage, and differentiation degree.