Protective Effects Of FLZ-52A, Salvianolic Acid A And Schisanhenol On Oxidative Stress Induced Injuries Of Cerebral Cells And Their Mechanisms

Oxidative stress refers to the cytological consequences of a mismatch between the production of free radicals and the ability of the cell to defend against them. A net increase in reactive oxygen species(ROS) can produce damage to lipids, proteins, and DNA and induce necrosis and apoptosis. Growing date from experiments models and human brain studies suggest that oxidative stress may play an important role in neuronal degeneration in diseases such as Parkinson's disease, Alzheimer's disease, and amyotophic lateral sclerosis. These disorders make the aged persons can not live normally and move freely. So it is necessary to find effective antioxidants to prevent or cure the aged men from the diseases related to neuronal degeneration. FLZ-52A is a synthetic analogue of a natural compound salvianolic acid A (Sal A) and schisanhenol (Sal) which were isolated from Salvia miltiorrhiza Bge (Dan Shen) and Kadsura Longipenduculata Finet et Gagnep (Hong Hua Wu Wei Zi) respectively. The three compounds were shown to have strong anti-lipid peroxidation activity. In this paper we have studied the protective effects of FLZ-52A, Sal A and Sal on oxidative stress induced injuries of cerebral cells in vitro and in vivo.1. Protective effect of FLZ-52A on oxidative stress induced injury of rat cerebral mitochondria and synaptosome.The aging brain undergoes a process of enhanced oxidative stress injury. Incubation of rat cerebral mitochondria or synaptosomes with Fe²⁺-cysteine(Cys) at 37℃ resulted in increase of malondiadehyde (MDA) formation and decrease of ATPase activity. FLZ-52A, Sal A or Sal (10^-4mol·L^-1) almost completely inhibited the peroxidative stress induced increase of MDA...