Effects Of G-CSF On Lipid Peroxidation And Neuronal Apoptosis In Rats Exposed To The Focal Cerebral Ischemia-reperfusion

ObjectiveTo provide further evidence for G-CSF used in the treatment of ischemic stroke by investigating its effects on lipid peroxidation and neuronal apoptosis in rats exposed to the focal cerebral ischemia-reperfusion.Methods18 male Wistar rats were randomly divided into 3 groups: sham operation group and the control group, and G-CSF group, 6 rats in each group. Modified suture method to establish the right middle cerebral artery occlusion (tMCAO). 2 hours after reperfusion, G-CSF group were given granulocyte colony-stimulating factor(50ug/kg)while the control group were injected with equivalent saline. 24h after reperfusion,the contents of MDA and NO in the ischemic region, as same as the activity of SOD and GSH-PX, were detected through chemical colorimetric method. p-p38MAPK and p-JNK expression was detected by immunohistochemical method. And then neuronal cell apoptosis was detected by tunel staining.Results24h after reperfusion, the activity of SOD and GSH-PX were higher in the G-CSF group than in the control group, but lower in the sham group (p <0.05), while MDA, NO levels lower in the control group but higher in the sham group (p <0.05); p-JNK and p-p38MAPK-positive cells were significantly less in the G-CSF group than in the ontrol group (p <0.05); apoptotic cells were found decreased in the G-CSF group compared with the control group by tunel staining.ConclusionG-CSF could significantly increase SOD, GSH-PX activities, reduce the level of lipid peroxidation, and depress neuronal apoptosis by antagonizing JNK, p38MAPK pathway.