Effects Of Antisense EGF And Antisense EGFR Retroviral Expression Vectors On The Cell Growth Of Human Pancreatic Cell Line

In this study, two new human pancreatic adenocarcinoma cell lines of PC-7 and PC-48, were established by adding soybean trypsin inhibitor to the culture medium.Up to now, the cell lines have been cultured continuously more than 150 (PC-7) and 50 (PC-48) passages in vitro. They were proved to be human pancreatic adenocarcinoma cell lines by morphological and immunocytochemical studies with anti-keratin and anti-CEA antisera. They shared common characteristics of short population doubling-time,high mitotic indices, and low diploid, abnormal chromosomal karyotypes.By immunocytochemical staining and Northern blot hybridization, expression of EGF and EGFR at proteins and mRNAs levels was detected in PC-7 and PC-48 cell lines, and compete-inhibit combination teBt of ¹²⁵I-EGF with EGF receptor demonstrated the presence of EGFR on PC-7 cell membrane. Growing slowly in low serum or serum-free medium,was accorded with the characteristic of reduced serum-dependence of PC-7.The effects of exogenous EGF, anti-EGF and anti-EGF receptor antisera treatment on the growth of PC-7 cell line in low-serum medium were: (1) EGF stimulated significantly the cell growth and hyperplaBia of PC-7, with high cell-count and high ³H-TdR incorporation rate as compared with the control; (2) Anti-EGF antiserum inhibited significantly the cell growth of PC-7, with low cell-count and low ³H-TdR incorporation rate. The cells grew slowly with irregular morphology (ghost cell), and poor adhesion, as compared with the control; (3)Anti-EGF receptor antiserum stimulated slightly the cell growth of PC-7, with slight increases of...