Studies On The Chemical Constituents And Bioactivities Of Medicinal Plant Beesia Calthaefolia (Maxim.) Ulhr. Native To China

Beesia calthaefolia (Maxim.) Ulbr. (Fam. Ranunculaceae) is widely distributed in southwest and northwest of China. As a Chinese folk herb medicine, it possesses anti-inflammatory, antipyretic, analgesic, antioncotic and detoxifying functions and can "invigorate blood circulation". Its rhizome or the whole plant is used to treat cold, rheumatic arthritis, dysentery, sore throat and headache etc. In order to obtain a more comprehensive understanding of its effective physiological constituents, in this doctoral dissertation, a systematic photochemical study has been carried out.Forty compounds were isolated from the ethanol extracts of its rhizome and whole plant native to Gansu and Guizhou provinces by various column chromatography. On the basis of various modern spectroscopic analysis (IR, UV, 1HNMR, 13CNMR, DEPT, 1H-1H COSY, 13C-1H COSY, HMQC, HMBC, TOCSY, HMQC-TOCSY, NOESY, EI-MS, FAB-MS, ESI-MS) and classical chemical methods, thirty three of them were determined, of which eighteen were new and twelve were isolated from the plant for the first time.Among the new compounds, there are sixteen cycloartane triterpenoid saponins, one oleanolic triterpenoid saponin and one organic acid. The structures of new compounds are as follows:beesioside A[BC-11, 20ε1-24ε2-epoxy-9,19-cyclolanostane-3β,16β,18,25-tetraol-3-O-β-D-xylopyranoside], beesioside B[BC-12, (20S*-24R*)-epoxy-9,19-cyclolanostane-3β,12β,16β, 25-tetraol-3-O-β-D-xylopyranoside], beesioside C[BC-10, (20S*,24R*)-16β-acetoxy-20,24-epoxy-9,19-cyclolanostane-3β, 12β,25-triol-3-O-β-D-xylopyranoside], beesioside D[BC-14, (20S*,24R*)-epoxy-9,19-cyclolanostane-3β,12β,16β,18,25-pentaol-3-O-β-D-xylopyranoside], beesioside E [BC-15, 20ε1-24ε2-epoxy-9,19-cyclolanostane-3β,12α, 16β, 18, 25-pentaol-3-O-β-D-xylopyranoside], beesioside F[BC-13, (20S*,24R*)-16β-acetoxy-20,24-epoxy-9,19-cyclolanostane-3β,12α, 18,25-tetraol-3-O-β-D-xylopyranoside], beesioside G[BC-16, 20ε1-24ε2-epoxy-9,19-cyclolanostane-3β, 15α, 16β, 18,25-pentaol-3-O-β-D-xylopyranoside], beesioside H[gbc-23, (20S.24R)-15α, 16β-diacetoxy-20, 24-epoxy-9,19-cyclolanostane-3β, 18, 25-triol-3-O-β-D-xylopyranoside], beesioside Ia[gbc-22, (20S, 24S)-16β-acetoxy-18, 24; 20, 24-diepoxy-9,19-cyclanostane-3β,15β, 25-triol-3-O-β-D-xylopyranoside], beesioside J[gbc-20, (20S, 24S)-16β-acetoxy-18,24; 20, 24-diepoxy-9,19-cyclanostane-3β, 25-diol-3-O-β-D-xylopyranoside], beesioside K[gbc-18, (20S, 24S)-15α-acetoxy-16β,24; 20,24-diepoxy-9,19-cyclolanostane-3β, 25-diol-3-O-β-D-xylopyranoside], beesioside L[gbc-19, 15α-acetoxy-20ε1-24ε2-epoxy-9,19-cyclolanostane-3β,16β,25-triol-3-O-β-D-xylopyranoside], beesioside M [gbc-26, (20S,24R)-15α-acetoxy-9,19-cyclolanostane-3β, 16β,20,24,25-pentaol-3-O-β-D-xylopyranoside], beesioside N[gbc-27, 20ε1-24ε2-epoxy-9,19-cyclolanostane-3β,12α,15α, 16β,25-pentaol-3-O-β-D-xylopyranoside], beesioside O[gbc-30, 20ε1-24ε2-epoxy-9,19-cyclolanostane-3β,16β,18,25-tetraol-3-O-β-D-glucopyranoside], beesioside P [gbc-31, 20ε1-...