| Repair of damaged tissues and organs is critical for the survival of any individual. The process begins with dead cells being replaced by vital ones. And then the vital cells must differentiate and become organized in complex patterns to restore the original structure of the injured tissue. Cerebral infarction is a very common disease nowadays, it threatens human's health seriously, but there are still no efficient ways to cure the disease. Transplanting neural stem cells (NSCs) to treat cerebral infarction maybe an attractive way, because neural stem cells can not only replace the dead cells in the infarction area but also differentiate into neurons, astrocytes and oligodendrocytes by them the origin tissue could be reconstructed again. In the experiments, we try to transplant NSCs to the peripheral of the infarction and hope that the grafts can survive in the penumbra and replace the dead cells gradually and reconstruct the damaged tissue in the end. Regarding the grafts are difficult to survive in the bad microenviroments of the infarction and its penumbra, a gene is transferred to NSCs to raise the survival rate of the grafts that can enhance angiogenesis and increase local blood flow. Four parts are designed in the experiments. Part one, the isolation, propagation and identification of neural stem cells from rat brain; part two, to make rat models of focal cerebral ischemia with suture method; part three, to transfer exogenous gene to NSCs by using recombinant adeno-associated virus type 2 ( rAAV2); part four, to transplant NSCs and transgenic NSCs to the penumbra of cerebral infarction. As results, we found that rAAV2 can transfect NSCs and exogenous report gene can express report...
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